肉豆蔻醚抑制结肠癌细胞增殖、迁移和侵袭的作用及机制研究  

The Mechanism of Myristicin Inhibiting Proliferation, Migration and Invasion of Colon Cancer Cell Lines

作　　者：段春燕[1] 何娜娜[1] 朱蕾 范磊 伊凡 王婷 邓皖利[5] Duan Chunyan;He Nana;Zhu Lei;Fan Lei;Yi Fan;Wang Ting;Deng Wanli(Affiliated Chinese Medicine Hospital of Xinjiang Medical University,Urumqi 830016,China;Xinjiang Changji Chinese Medicine Hospital,Changji 831100,China;Qingdao West Coast New District Central Hospital,Qingdao 266555,China;Urumqi Chinese Medicine Hospital,Urumqi 830000,China;Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200120,China)

机构地区：[1]新疆医科大学附属中医医院,乌鲁木齐830016 [2]新疆昌吉州中医医院,昌吉831100 [3]青岛西海岸新区中心医院,青岛266555 [4]乌鲁木齐市中医医院,乌鲁木齐830000 [5]上海中医药大学附属曙光医院,上海200120

出　　处：《世界科学技术-中医药现代化》2020年第4期907-913,共7页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：新疆维吾尔自治区科技厅新疆自治区科技支疆项目(2013911125):维药沙棘肉豆蔻组方抗大肠癌EMT的活性物质及其机制研究,负责人:邓皖利

摘　　要：目的探讨肉豆蔻醚(myristicin)对结肠癌细胞增殖、迁移和侵袭的作用及机制。方法MTT法检测肉豆蔻醚在体外对结肠癌细胞HCT116和LOVO增殖的影响;Annexin V/PI双染法检测肉豆蔻醚对HCT116和LOVO细胞凋亡的影响;划痕实验和Transwell实验分别检测肉豆蔻醚对HCT116和LOVO细胞迁移和侵袭的影响;western blot实验检测肉豆蔻醚对p-MEK1/2、p-ERK1/2、CyclinD1、E-cadherin、MMP-2以及MMP-9表达的影响。结果肉豆蔻醚作用后,HCT116和LOVO细胞增殖、迁移和侵袭受到显著抑制,细胞出现显著凋亡;肉豆蔻醚作用后,HCT116和LOVO细胞内MEK1/2、ERK1/2、CyclinD1、MMP-2以及MMP-9蛋白表达水平显著降低,而E-cadherin蛋白表达水平显著增高。结论本研究发现肉豆蔻醚在体外具有抑制结肠癌细胞生长的作用,为临床用药提供一定的实验依据,为肿瘤治疗提供新的靶点和策略。Objective To investigate the effect and molecular mechanism of myristicin on proliferation migration and invasion of colon cnacer cell lines(HCT116 and LOVO).Methods The effect of myristicin on proliferation of colon cancer cells HCT116 and LOVO in vitro was detected by MTT assay.The apoptosis of myristicin on HCT116 and LOVO cells was detected by Annexin V/PI.The migration and invasion ability of myristicin were detected by wound healing and Transwell assay.The effect of myristicin on the expression of MEK1/2,ERK1/2,CyclinD1,E-cadherin,MMP-2 and MMP-9 was analysised by Western blot.Results The proliferation,migration and invasion of HCT116 and LOVO cells were significantly inhibited by myristicin.After myristicin treatment,the expression of p-MEK1/2,p-ERK1/2,CyclinD1,MMP-2,and MMP-9 were significantly decreased in HCT116 and LOVO cells,while the expression of Ecadherin protein was significantly increased.Conclusion Myristicin could inhibit the growth of colon cancer cells in vitro,which provided a certain experimental basis for clinical drug use,and provided new targets and strategies for cancer treatment.

关 键 词：肉豆蔻醚 结肠癌 凋亡 迁移 侵袭 

分 类 号：R735.3[医药卫生—肿瘤]