石蒜碱内铵盐和细小病毒H-1对胃癌细胞杀伤作用的实验研究  

THE CYTOCIDAL EFFECT OF AT-1840 AND PARVOVIRUS H-1 ON GASTRIC CANCER CELLS

作　　者：吴云林 吴裕炘 余春仙 张素胤[2] 苏兆众[3] 江绍基[1] 

机构地区：[1]上海市消化疾病研究所 [2]中国科学院上海药物研究所 [3]复旦大学生物系

出　　处：《上海医学》1988年第12期683-688,2,共7页Shanghai Medical Journal

摘　　要：体外三种胃癌细胞株的染料排斥试验及~3H—胸腺嘧啶掺入试验均证实石蒜碱内铵盐(AT-1840)及细小病毒H-1(PVH-1)对胃癌细胞具有直接杀伤作用,药物浓度与杀伤强度呈正比,最低有效浓度分别为0.1μg/孔和1×10~4/孔。流式细胞仪检测证实AT-2840作用于G2/M增殖期胃癌细胞。PVH-1作用于S期胃癌细胞。PVH-1感染肿瘤细胞后即掺入细胞核内增殖,细胞膜绒毛变性、丢失,其后细胞肿胀、崩解。两药最低有效剂量联用,对胃癌细胞杀伤作用起协同增强效应,抗肿瘤作用可超过任一单独应用最大剂量时的作用强度。AT-1840使实体瘤重量明显减少,PVH-1和AT-1840明显延长带胃癌裸小鼠(腹水型及实体型)的生存期。本研究首次证实AT-1840和PVH-1均为低浓度有效的胃癌细胞直接杀伤因子,且具有协同增强作用。This study tried to demonstrate the in vitro killing effect of AT-1840(a Chinese herbal extract)on gastric cancer cells and also to identify the sites of action on cell cycle of these cells.The dye exclusion test and~3H-thymidine incorporation have demonstrated that AT-1840 and parvovirus H-1(PVH-1)have direct cytocidal effect on stomach cancer cells.Combined use of AT-1840 and PVH-1 at the lowest concentrations increased the.cytocidal effect markedly.Using flow cytometry,it was found that both drugs were cell cycle specific agents,there was high accumulation of G/M phase cells for AT-1840 and S phase cells for PVH-1.With transmission and scanning electron mic(?)oscopy,it was found that once cancer cells were infected with PVH-1,this virus could penetrate into the nuclei of cancer cells and replicated,and resulted in the shortening of cancer cells villi and cell lysis ensued.In stomach cancer-bearing nude mice,both A T-1840 and PV H-1 prolonged the survival period and decreased the size of solid tumors.

关 键 词：胃癌细胞 染料排斥试验 H-1 杀伤作用 细小病毒 石蒜碱 抗阿米巴药 铵盐 

分 类 号：R73[医药卫生—肿瘤]