L[1-13C]-亮氨酸示踪测定烫伤兔总体蛋白质动力学  

STUDY OF TOTAL BODY PROTEIN KINETICS IN SCALDED RABBITS USING L-(1-13C)-LEUCINE TRACING TECHNIQUE

作　　者：张小俊 方培跃[1] 史济湘[1] 周文达[2] 夏宗勤[2] 徐信容[3] 黄关禄 鲁一江 李晶 史爱琴 

机构地区：[1]上海第二医科大学附属瑞金医院烧伤科 [2]上海第二医科大学同位素研究室 [3]上海第二医科大学色质谱室 [4]上海测试技术研究所气体室 [5]上海第二医科大学附属瑞金医院肺功能室

出　　处：《上海医学》1988年第5期288-291,共4页Shanghai Medical Journal

摘　　要：作者用L-[1-13C]-亮氨酸体内示踪的方法测定6只烫伤兔总体蛋白质动力学变化。结果表明兔在受到15%三度烫伤后48小时,总体蛋白质代谢的流量(Q)、合成速度(S)和分解速度(C)分别是对照兔的2、2.2和2.6倍。烫伤兔Q、S、C都与摄入蛋白量(I)有正相关性(r=0.98,P<0.01;r=0.92,P<0.01;r=0.83,P<0.05),对照兔仅Q与I,S与I之间有相关性(r=0.89,P<0.05;r=0.81,P<0.05),C与I无显著相关(r=-0.16,P>0.05)。两组动物随着摄入蛋白量增加,净蛋白合成率线性上升(r=0.97,P<0.01;r=0.92,P<0.01)。Using L-(1-13C)-leucine tracing technique,we determined the total body protein kinetics in 6 rabbits with third degree burn of 15%BSA and 6 normal rabbits.The results showed that 48 hours after scalding,the total body protein metabolic flux(Q),synthesis(S),and catabolism(C)were 2.0-,2.2-,and 2.6-fold those in normal rabbits respectively.In scalded rabbits,there were positive correlations between Q and I,S and I,and C and I,while in normal ones positive correlations were found only between Q and I,and S and I.Moreover,with increased protein intake,net protein synthesis rose parallelly in both groups.When normal rabbits were given protein>3.2g/kg/d,positive nitrogen balance could be expected,however the scalded rabbits showed negative nitrogen balance regardless of high protein intake,suggesting high protein intake could not reverse negative nitrogen balance in scalded rabbits.

关 键 词：蛋白质代谢 蛋白量 分解速度 合成速度 蛋白代谢 亮氨酸 白氨酸 L[1 摄入 蛋白合成 

分 类 号：R28[医药卫生—中药学]