干扰MAD2L1基因表达对乳腺癌细胞凋亡的影响及机制  

Effects and mechanism of interfering MAD2L1 gene expression on the apoptosis of breast cancer cells

作　　者：封海岗 刘国文 曹洪 FENG Haigang;LIU Guowen;CAO Hong(Department of Thyroid and Breast Surgery,The Second Affiliated Hospital,Hengyang Medical School,University of South China,Hengyang 421001,Hunan,China;Department of Thyroid and Breast Surgery,Shenzhen Second People's Hospital,Shenzhen 518025,Guangdong,China)

机构地区：[1]南华大学衡阳医学院附属第二医院乳甲外科,湖南衡阳421001 [2]深圳市第二人民医院甲乳外科,广东深圳518025

出　　处：《山东大学学报（医学版）》2022年第10期9-16,共8页Journal of Shandong University:Health Sciences

基　　金：湖南省卫生健康委课题(202204013935);湖南省自然科学基金(2018JJ2354);衡阳市科技局指导性项目(202121034412)

摘　　要：目的探讨干扰有丝分裂阻滞缺陷2样蛋白1(MAD2L1)基因表达对乳腺癌(BC)细胞凋亡的影响及其机制。方法采用qRT-PCR检测正常乳腺上皮细胞系MCF-10A和4种BC细胞系(MDA-MB-231、MCF-7、SK-BR-3和BT-20)中MAD2L1 mRNA表达水平。将MAD2L1 siRNA转染至MDA-MB-231细胞中,qRT-PCR和Western blotting检测细胞中MAD2L1 mRNA和蛋白表达水平;MTT检测细胞增殖能力;流式细胞术法检测细胞凋亡率;Western blotting检测细胞中Bax、Bcl-2、cleaved-caspase-3、p-p38MAPK(Thr180/Thr182)和p38MAPK等蛋白表达水平。采用p38MAPK抑制剂SB203580联合处理上述细胞,Annexin V-FITC/PI法检测细胞凋亡率的变化;Western blotting检测Bax、Bcl-2、cleaved-caspase-3、p-p38MAPK和p38MAPK表达水平的变化。结果BC细胞系中MAD2L1 mRNA表达水平高于MCF-10A细胞,其中MDA-MB-231细胞最为显著。干扰MAD2L1基因可降低MDA-MB-231细胞中MAD2L1 mRNA和蛋白表达水平(t_(mRNA)=10.51,P_(mRNA)<0.001;t_(蛋白)=18.30,P_(蛋白)<0.001),同时抑制细胞增殖(F_(组别)=243.36、F_(时间)=44.00、F_(组别×时间)=9.881,P均<0.001),促进细胞凋亡(t=9.10,P<0.001),并上调Bax、cleaved-caspase-3和p-p38MAPK蛋白表达水平(t_(Bax)=15.05,P_(Bax)<0.001;t_(cleaved-caspase-3)=5.26,P_(cleaved-caspase-3)=0.006;t_(p-p38MAPK)=28.46,P_(p-p38MAPK)<0.001),下调Bcl-2蛋白表达水平(t_(Bcl-2)=14.23,P<0.001)。然而,SB203580处理可抑制MAD2L1基因干扰对MDA-MB-231细胞凋亡的诱导作用(P=0.002)。结论干扰MAD2L1基因表达可抑制MDA-MB-231细胞增殖,并诱导其凋亡,其作用机制可能与激活p38MAPK信号通路有关。Objective To explore the effects of interference with mitotic arrest deficient 2-like protein 1(MAD2L1)gene expression on breast cancer(BC)cell apoptosis and the mechanism.Methods The mRNA expressions of MAD2L1 in normal breast epithelial cell line MCF-10 A and BC cell lines,including MDA-MB-231,MCF-7,SK-BR-3 and BT-20,were detected with qRT-PCR.After MAD2L1 siRNA was transfected into MDA-MB-231 cells,the mRNA and protein expressions of MAD2L1 were detected with qRT-PCR and Western blotting;cell proliferation ability was measured with MTT;cell apoptosis was detected with Annexin V-FITC/PI;expressions of Bax,Bcl-2,cleaved-caspase-3,p-p38 MAPK(Thr180/Thr182)and p38 MAPK were determined with Western blotting.After the above cells were treated with the p38 MAPK inhibitor SB203580 in combination,the changes in apoptosis rate were detected with flow cytometry,and changes in the expressions of Bax,Bcl-2,cleaved-caspase-3,p-p38 MAPK and p38 MAPK were detected with Western blotting.Results The mRNA expression of MAD2L1 in BC cell line,especially in MDA-MB-231 cells,was significantly higher than that in MCF-10 A cells.Interference with MAD2L1 gene reduced the mRNA and protein expressions of MAD2L1 in MDA-MB-231 cells(t_(mRNA)=10.51,P_(mRNA)<0.001;t_(protein)=18.30,P_(protein)<0.001),inhibited cell proliferation(F_(group)=243.36,F_(time)=44.00,F_(group×time)=9.881,all P<0.001),promoted cell apoptosis(t=9.10,P<0.001),up-regulated the protein expressions of Bax,cleaved-caspase-3 and p-p38 MAPK(t_(Bax)=15.05,P_(Bax)<0.001;t_(cleaved-caspase-3)=5.26,P_(cleaved-caspase-3)=0.006;t_(p-p38 MAPK)=28.46,P_(p-p38 MAPK)<0.001),and down-regulated the protein expression of Bcl-2(t_(Bcl-2)=14.23,P_(Bcl-2)<0.001).However,SB203580 treatment inhibited the induction of apoptosis by MAD2L1 gene interference on MDA-MB-231 cells(P=0.002).Conclusion Interfering with the expression of MAD2L1 gene can inhibit the proliferation of MDA-MB-231 cells and induce apoptosis,and the mechanism may be related to the activation of the p38 MAPK signaling path

关 键 词：乳腺癌 有丝分裂阻滞缺陷2样蛋白1 细胞凋亡 P38MAPK信号通路 

分 类 号：R737.9[医药卫生—肿瘤]