牛磺酸对1-溴丙烷致大鼠认知功能障碍的保护作用  

Protective effect of taurine on cognitive deficit induced by 1-bromopropane

作　　者：赵慧文 许琳 单姗 赵秀兰 ZHAO Huiwen;XU Lin;SHAN Shan;ZHAO Xiulan(Department of Toxicology and Nutrition,School of Public Health,Shandong University,Jinan 250012,Shandong,China)

机构地区：[1]山东大学公共卫生学院卫生毒理与营养学系,山东济南250012

出　　处：《山东大学学报（医学版）》2022年第2期14-21,共8页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(81872654)

摘　　要：目的观察牛磺酸对1-溴丙烷致大鼠中枢神经系统功能损伤的保护作用。方法SPF级成年雄性Wistar大鼠60只,按体质量随机分为空白对照组、1-溴丙烷染毒组、低剂量牛磺酸干预组、高剂量牛磺酸干预组、牛磺酸对照组。1-溴丙烷采用玉米油稀释,经口染毒,剂量800 mg/kg,染毒15~20 d,采用Morris水迷宫检测大鼠认知功能。实验21 d处死动物,迅速分离大脑皮层及海马,采用高效液相色谱法检测脑组织中牛磺酸含量,采用Western blotting法检测线粒体氧化磷酸化蛋白复合体及凋亡相关蛋白的表达。结果Morris水迷宫结果显示,与空白对照组相比,1-溴丙烷染毒组大鼠逃避潜伏期延长,穿越平台次数明显降低(P<0.01);与1-溴丙烷染毒组相比,低剂量和高剂量牛磺酸干预组逃避潜伏期明显缩短,穿越平台次数增加(P<0.01)。HPLC结果显示,与空白对照组相比,1-溴丙烷染毒组大鼠皮层和海马牛磺酸含量明显降低(P<0.001,P<0.05),与1-溴丙烷染毒组相比,低剂量和高剂量牛磺酸干预组大鼠脑组织牛磺酸含量明显升高(P<0.001,P<0.01)。与空白对照组相比,1-溴丙烷染毒组大鼠脑组织中线粒体氧化磷酸化蛋白复合体和Bcl-2表达明显降低(P<0.01,P<0.05),BAX和cleaved caspase-3表达升高(P<0.05,P<0.01);低剂量和高剂量牛磺酸干预组大鼠脑组织线粒体氧化磷酸化蛋白复合体及凋亡相关蛋白的变化趋势有明显逆转,与1-溴丙烷染毒组相比,差异均有统计学意义(P<0.01,P<0.05,P<0.001)。结论补充牛磺酸可抑制1-溴丙烷诱导大鼠大脑的神经细胞凋亡,维持大脑线粒体正常功能,进而改善1-溴丙烷致大鼠的认知功能障碍。Objective To explore the protective effect of taurine on cognitive deficit induced by 1-bromopropane(1-BP).Methods A total of 60 SPF adult male Wistar rats were randomly divided into control group,1-BP group,low-dose taurine intervention group,high-dose taurine intervention group and taurine control group according to body weight.1-BP was dissolved in corn oil and orally administrated at the dose of 800 mg/kg.bw.The spatial learning and memory function of rats were detected with Morris water maze(MWM)15-20 days after the administration of 1-BP.On Day 21,the rats were sacrificed and the cerebral cortex and hippocampus were separated immediately.The content of taurine was determined with high-performance liquid chromatography(HPLC).The expressions of oxidative phosphorylation protein complex of mitochondria and apoptosis related proteins in brains were detected with Western blotting.Results Morris water maze showed that compared with the control group,1-BP group had longer escape latency,and decreased frequencies of crossing platform(P<0.01);compared with 1-BP group,the low-dose and high-dose taurine intervention groups had shorter escape latency and increased frequencies of crossing platform(P<0.01).The HPLC results showed that compared with the control group,the 1-BP group had significantly decreased taurine contents in cerebral cortex and hippocampus(P<0.001,P<0.05);compared with 1-BP group,the low-dose and high-dose taurine intervention groups had significantly increased taurine level(P<0.001,P<0.01).Compared with the control group,the 1-BP group had significantly decreased expression of mitochondrial oxidative phosphorylation protein complex(OXPHOS)and Bcl-2(P<0.01,P<0.05),but significantly increased expressions of BAX and cleaved capase-3(P<0.05,P<0.01).The alteration trends of OXPHOS and apoptosis related proteins in low-dose and high-dose taurine intervention groups were significantly reversed(P<0.01,P<0.05,P<0.001).Conclusion Taurine supplementation could inhibit neuronal apoptosis,maintain the normal func

关 键 词：1-溴丙烷 牛磺酸 大鼠 认知功能障碍 

分 类 号：R285.5[医药卫生—中药学]