CDC7、MCM4在105例宫颈病变组织中的表达及意义  

Expressions and significance of CDC7 and MCM4 in 105 cases of cervical lesions

作　　者：陈泉材 韩赛[1] 刘露 孙雨 尤学武 张俊华 张友忠[1,2] CHEN Quancai;HAN Sai;LIU Lu;SUN Yu;YOU Xuewu;ZHANG Junhua;ZHANG Youzhong(Department of Obstetrics and Gynecology,Qilu Hospital of Shandong University,Jinan 250012,Shandong,China;Cheeloo College of Medicine,Shandong University,Jinan 250012,Shandong,China)

机构地区：[1]山东大学齐鲁医院妇产科,山东济南250012 [2]山东大学齐鲁医学院,山东济南250012

出　　处：《山东大学学报（医学版）》2022年第1期34-39,共6页Journal of Shandong University:Health Sciences

基　　金：国家重点研发计划(2016YFC1302900);国家自然科学基金(81572559);山东省重点研究计划(2017CXGC1210);山东省科学技术发展计划(2014GGH218029)

摘　　要：目的探讨细胞分裂周期蛋白7(CDC7)、微小染色体维持蛋白4(MCM4)在宫颈癌及癌前病变中的表达及临床意义。方法利用免疫组织化学检测CDC7、MCM4蛋白在30例正常宫颈、30例宫颈上皮内瘤变I级(CINⅠ)、25例CINⅡ~Ⅲ、50例宫颈癌组织标本中的表达,与传统细胞增殖标记物Ki67比较,分析其与宫颈癌临床病理特征的关系。结果①CDC7在正常宫颈、CINⅠ、CINⅡ~Ⅲ及宫颈癌组织中的阳性表达率分别为6.7%、26.7%、56.0%和78.0%,表达水平与病变程度呈中度正相关(r_(s)=0.625),差异有统计学意义(P<0.001);MCM4在4组中的阳性表达率分别为26.7%、36.7%、64.0%和88.0%,表达水平与病变程度呈中度正相关(r_(s)=0.680),差异有统计学意义(P<0.001);Ki67在4组中阳性表达率分别为10.0%、26.7%、60.0%和84.0%,表达水平与病变程度呈中度正相关(r_(s)=0.660),差异有统计学意义(P<0.001);②CDC7蛋白表达水平与宫颈癌病理分级、淋巴转移和肌层浸润深度相关(P=0.024、P=0.033、P=0.023),MCM4蛋白表达水平与宫颈癌病理分级和肌层浸润深度相关(P=0.010、P=0.003),Ki67蛋白表达水平与宫颈癌病理分级、淋巴转移和肌层浸润深度相关(P=0.009、P=0.016、P=0.021);③CDC7与MCM4在宫颈癌组织中的表达水平呈正相关(P=0.001);④多因素调整后CDC7、MCM4、Ki67蛋白的阳性表达是宫颈组织病变严重程度的危险因素。结论CDC7、MCM4蛋白在CIN及宫颈癌中高表达,具有成为CIN及宫颈癌诊疗参考指标的潜在临床价值。Objective To explore the expressions and clinical significance of cell division cycle 7(CDC7)and minichromosome maintenance protein 4(MCM4)in cervical cancer and precancerous lesions.Methods The expressions of CDC7 and MCM4 in 30 cases with normal cervix,30 cases with grade I cervical intraepithelial neoplasia(CINⅠ),25 cases with CINⅡ-Ⅲ,and 50 cases with cervical cancer were detected with immunohistochemistry.The expressions were compared with Ki67,and their correlation with the clinicopathological characteristics of cervical cancer was analyzed.Results①The positive expression rate of CDC7 in normal cervical tissues,CINⅠ,CINⅡ-Ⅲ,and cervical cancer tissues was 6.7%,26.7%,56.0%and 78.0%,respectively,which was moderately and positively correlated with the lesion degree(r_(s)=0.625,P<0.001).The positive expression rate of MCM4 in the 4 groups was 26.7%,36.7%,64.0%and 88.0%,respectively,which was moderately and positively correlated with the lesion degree(r_(s)=0.680,P<0.001).The positive expression rate of Ki67 in the 4 groups was 10.0%,26.7%,60.0%and 84.0%,respectively,which was moderately and positively correlated with the lesion degree(r_(s)=0.660,P<0.001).②The positive expression level of CDC7 was correlated with the pathological grade,lymphatic metastasis and depth of muscular invasion of cervical cancer(P=0.024,P=0.033,P=0.023).The positive expression level of MCM4 was correlated with the pathological grade and depth of muscular invasion of cervical cancer(P=0.010,P=0.003).The positive expression level of Ki67 was correlated with the pathological grade,lymphatic metastasis and depth of muscular invasion of cervical cancer(P=0.009,P=0.016,P=0.021).③The expression levels of CDC7 and MCM4 were positively correlated in cervical cancer tissues(P=0.001).④After multivariate adjustment,the positive expressions of CDC7,MCM4 and Ki67 were risk factor for the severity of cervical lesions.Conclusion CDC7 and MCM4 are highly expressed in CIN and cervical cancer,which suggests that they have potential cli

关 键 词：宫颈癌 宫颈上皮内瘤变 CDC7蛋白 MCM4蛋白 KI67蛋白 

分 类 号：R737.33[医药卫生—肿瘤]