基于导数-恒基体同步荧光的纺织品中萘胺同分异构体快速定量检测  

Rapid quantitative detection of naphthylamine isomers in textiles based on Derivative-Matrix Isopotential Synchronous Fluorescence

作　　者：李娜[1] 魏嘉雯 吴平平[1] 赵妍[1] 谢堂堂 李耀群[1] LI Na;WEI Jia-wen;WU Ping-ping;ZHAO Yan;XIE Tang-tang;LI Yao-qun(Xiamen University,Xiamen,Fujian,361005;Shenzhen Customs and Quarantine Bureau Industrial Products Testing Technology Centre,Shenzhen,Guangdong,518067)

机构地区：[1]厦门大学,福建厦门361005 [2]深圳海关工业品检测技术中心,广东深圳518067

出　　处：《生命科学仪器》2023年第5期31-37,共7页Life Science Instruments

基　　金：福建省科技计划项目(项目号:2022Y4008);国家自然科学基金(项目号:22274137)资助

摘　　要：2-萘胺是纺织品中禁用的一种致癌芳香胺,其与同分异构体1-萘胺(非禁用)的结构、性质相似,传统检测方法在检测纺织品实际样品时可能会造成阳性结果的误判,因此相关检测标准要求采用多种方法对阳性结果进行复查确认。目前常规的复查方法为气相色谱-质谱联用法和高效液相色谱法,存在操作复杂、耗时长等弊端,因此开发一种简单、快速的方法用于鉴别和定量纺织品中的致癌芳香胺及其异构体具有重要意义。导数-恒基体同步荧光法具有灵敏度高、选择性好、可消除背景干扰等特点,已广泛地应用于复杂基质中物质的测定。为了实现纺织品中1-萘胺(1-NA)和2-萘胺(2-NA)的快速鉴别定量,建立了一种导数-恒基体同步荧光分析法。研究确立了一条合适的恒基体同步荧光扫描路径,提高1-NA和2-NA的光谱分辨率;结合一阶导数技术消除背景干扰,得到1-NA和2-NA的净信号。以上两种技术的结合,能够保持较高的灵敏度和选择性,同时避免了耗时的物理分离过程且无需多次扫描。整个光谱的扫描过程可在2分钟内完成。实验结果表明,1-NA和2-NA的检出限分别为0.001 mg/L(相当于纺织品中的0.1 mg/kg)和0.006 mg/L(相当于纺织品中的0.6 mg/kg),远远低于国家标准中规定的限量(20 mg/kg);1-NA的加标回收率在89.1~122.1%之间,2-NA的加标回收率在88.5~111.0%之间,相对标准偏差均小于9.3%;将所提出的方法与HPLC法作统计比较,得到的测试结果之间无显著差异。因此,所提出的方法具有较高的选择性、灵敏度,能够准确、可靠地鉴别和定量2-NA及其同分异构体1-NA,可应用于纺织品中禁用芳香胺的实际检测。2-naphthylamine(2-NA)is a prohibited carcinogenic aromatic amine in textiles.It is similar in structure and properties to the isomer 1-naphthylamine(1-NA)which is non-prohibited,while traditional detection methods may cause false positive results when testing textile sample.The relevant standards require the use of multiple methods for re-examination and confirmation when a positive result is detected.And the current conventional re-examination methods are gas chromatography-mass spectrometry and high performance liquid chromatography,which have disadvantages such as complicated operation and time-wasting.It is of great importance to develop a simple and rapid method for the identification and quantification of carcinogenic aromatic amines and their isomers in textiles.Derivative matrix isopotential synchronous fluorescence spectroscopy has the characteristics of high sensitivity,good selectivity and elimination of background interference,and is widely used in the determination of substances in complex samples.In order to realize the rapid identification and quantification of 1-NA and 2-NA in textiles,a synchronous fluorescence analysis method combined with derivative technology was established.Establishing a proper scanning trajectory of matrix isopotential synchronous fluorescence spectroscopy(MISFS)can improve spectral resolution of isomers.When MISFS combined with first derivative technique,the background interference is eliminated and the sensitivity and selectivity are both improved.Meanwhile,time-wasting physical separation process and multi-scanning are avoided.The scanning time is about 2 min.According to the experiment results,the detection limits for 1-NA and 2-NA are 0.001 mg/L(corresponding to 0.1 mg/kg in textiles)and 0.006 mg/L(corresponding to 0.6 mg/kg in textiles),respectively.And the spiked recoveries of 1-NA and 2-NA are 89.1-122.1%and 88.5-111.0%with the relative standard deviation less than 9.3%,respectively.Comparing the proposed method with HPLC,the test results of above two methods show n

关 键 词：禁用芳香胺 同分异构体 恒基体同步荧光法 导数技术 快速检测 

分 类 号：O657.3[理学—分析化学]