结肠癌患者VEGF和Ki-67及TRPC6的表达及与预后的相关性  

Expression of VEGF,Ki-67 and TRPC6 in patients with colon cancer and correlation with prognosis

作　　者：何进 徐月 沈文婷 马春燕 HE Jin;XU Yue;SHEN Wenting;MA Chunyan(Department of Pathology,Jiangsu Shengze Hospital,Nanjing Medical University,Shengze,Jiangsu 215000,China)

机构地区：[1]南京医科大学附属江苏盛泽医院病理科,江苏盛泽215000

出　　处：《热带医学杂志》2023年第11期1554-1557,共4页Journal of Tropical Medicine

基　　金：江苏省卫生健康委科研项目(Z2021090);江苏盛泽医院科研项目(SYK201813)

摘　　要：目的探讨结肠癌患者血管内皮生长因子(VEGF)、Ki-67蛋白及瞬时受体电位阳离子通道蛋白6(TRPC6)的表达及与预后的相关性。方法选择2016年1月-2022年7月南京医科大学附属江苏盛泽医院收治的70例结肠癌患者的组织标本进行研究,设为病例组,根据预后情况分为死亡组20例,存活组50例,同期选取本院病理科收集且经病理学检查证实非结肠癌的标本60例设为对照组。采用免疫组化染色分析两组患者VEGF、Ki-67及TRPC6阳性表达率及与临床病理、预后之间的关系。结果病例组患者VEGF的阳性为82.86%(58/70),明显高于对照组的13.33%(8/60);病例组患者Ki-67的阳性为85.71%(60/70),明显高于对照组的3.33%(2/60),病例组患者TRPC6的阳性为80.00%(56/70),明显高于对照组的5.00%(3/60),差异均有统计学意义(χ^(2)=62.479、87.891、73.317,P均<0.05)。病例组患者不同性别、年龄及分化程度的VEGF、Ki-67及TRPC6表达水平比较差异无统计学意义(P>0.05);不同肿瘤直径、淋巴结转移、临床分期情况的VEGF、Ki-67及TRPC6阳性表达率比较差异均有统计学意义(χ_(肿瘤直径)^(2)=5.398、6.459、4.644,χ_(淋巴结转移)^(2)=5.013、4.039、6.058,χ_(临床分期)^(2)=9.272、20.000、19.659,P均<0.05);死亡组VEGF、Ki-67及TRPC6阳性表达率(100.00%、100.00%、100.00%)均显著高于存活组(76.00%、80.00%、80.00%),差异均有统计学意义(χ^(2)=5.793、4.667、4.667,P均<0.05);相关性分析显示,VEGF、Ki-67及TRPC6与结肠癌患者肿瘤直径、淋巴结转移、临床分期及预后之间均成正相关(r_(VEGF)=0.511、0.499、0.466、0.536,r_(Ki-67)=0.487、0.526、0.476、0.584,r_(TRPC6)=0.605、0.551、0.399、0.635,P均<0.05)。结论结肠癌组织中VEGF、Ki-67及TRPC6阳性表达率异常,可能协同参与了结肠癌的发生发展,可预测患者预后情况。Objective To analyze the expression of vascular endothelial growth factor(VEGF),Ki-67 protein and transient receptor potential cation channel protein 6(TRPC6)in patients with colon cancer and their correlation with prognosis.Methods A total of 70 patients with colon cancer admitted to our hospital from January 2016 to July 2022 were selected as case group.According to the prognosis,20 patients were divided in the death group and 50 patients in the survival group.The tissues of 60 cases collected from the Pathology Department of our hospital and confirmed by pathological examination to be non-colon cancer specimens were selected as control group.Immunohistochemical staining was used to analyze the positive expression rates of VEGF,Ki-67 and TRPC6 and their relationship with clinicopathology and prognosis.Results The positive rate of VEGF in case group was 82.86%(58/70),which was significantly higher than that in control group 13.33%(8/60).The positive rate of Ki-67 in the case group was 85.71%(60/70),which was significantly higher than that in the control group 3.33%(2/60).The positive rate of TRPC6 in the case group was 80.00%(56/70),which was significantly higher than that in the control group 5.00%(3/60).The differences were statistically significant(χ^(2)=62.479,87.891,73.317;all P<0.05).There were no significant differences in the expression levels of VEGF,Ki-67 and TRPC6 among patients with different gender,age and differentiation degree in the case group(P>0.05).There were statistically significant differences in the positive expression rates of VEGF,Ki-67 and TRPC6 among different tumor diameters,lymph node metastasis and clinical stage(χ^(2)_(tumor size)=5.398,6.459,4.644,χ^(2)_(lymph node metastasis)=5.013,4.039,6.058,χ^(2)_(clinical stage)=9.272,20.000,19.659;all P<0.05).The positive expression rates of VEGF,Ki-67 and TRPC6 in death group(100.00%,100.00%,100.00%)were significantly higher than those in survival group(76.00%,80.00%,80.00%)(χ^(2)=5.793,4.667,4.667;all P<0.05).Correlation analysis show

关 键 词：结肠癌 血管内皮生长因子 KI-67蛋白 瞬时受体电位阳离子通道蛋白6 

分 类 号：R735.35[医药卫生—肿瘤]