结直肠癌组织中GPM6A的表达及其临床意义  

The expression and clinical significance of GPM6A in colorectal cancer tissues

作　　者：武彤彤 何清莲[2] 蔡方 许萍榕[1] 邹倩 朱伟 WU Tong-tong;HE Qing-lian;CAI Fang;XU Ping-rong;ZOU Qian;ZHU Wei(Department of Pathology,Department of Pathology Technology,Guangdong Medical University,Dongguan,Guangdong 523808,China;Department of Pathology,Huizhou Central People's Hospital,Huizhou,Guangdong 516001,China)

机构地区：[1]广东医科大学病理学系,病理学技术教研室,广东东莞523808 [2]惠州市中心人民医院病理科,广东惠州516001

出　　处：《热带医学杂志》2022年第10期1325-1329,1320,共6页Journal of Tropical Medicine

基　　金：国家自然科学基金(81472275);广东省自然科学基金(2020A151501303,2022A1515012171);广东省普通高校特色创新项目(2020KTSCX045);广东医科大学科研基金自然科学类重点培育项目(GDMUZ2020001)

摘　　要：目的研究糖蛋白M6A(GPM6A)在结直肠癌(CRC)组织中的表达,探讨GPM6A作为关键因子在CRC循环肿瘤细胞(CTCs)中的潜在价值。方法采用免疫组化法分别检测GPM6A在70例原发性CRC组织及癌旁正常组织的表达水平。利用GEPIA2分析TCGA数据库中GPM6A在CRC不同分期中的表达水平,利用在线数据库ctcRbase获得GPM6A在CRC原发灶、转移灶及CTCs中的表达水平。最后分析GPM6A与CRC细胞上皮-间质转变(EMT)功能状态相关基因的关系。结果GPM6A在CRC中高表达,在癌旁正常组织中低表达或不表达(P<0.001);GPM6A的表达随着肿瘤分期升高而升高(F=2.87,P=0.036)。相较于CRC原发灶和转移灶,GPM6A在CTCs中的表达最高。GPM6A与CRC细胞EMT状态相关基因半乳糖苷酶β1样2(GLB1L2)成负相关(r=-0.13,P<0.05),而与Ⅳ型胶原α2链(COL4A2)、补C1s(C1S)、I型胶原α2链(COL1A2)和钙桥1(CALD1)成正相关(r=0.26、0.43、0.21、0.67,P均<0.05)。结论GPM6A是驱使CRC进展的促癌因子,且GPM6A可能是CRC细胞发生EMT的关键因子,其高水平的表达使GPM6A有可能成为监测CRC复发的生物学标志物。Objective To investigate the expression of glycoprotein M6A(GPM6A)in colorectal cancer(CRC)and explore the potential value of GPM6A as a key factor in circulating tumor cells(CTCs)of CRC.Methods The expression of GPM6A was detected by immunohistochemistry in 70 cases of primary CRC and adjacent normal tissues.The expression level of GPM6A in different stages of CRC in TCGA database was analyzed by GEPIA2,and the expression levels of GPM6A in primary CRC,metastatic CRC and CTCs were obtained by online database ctcRbase.Finally,the relationship between GPM6A and the genes related to the functional status of epithelial mesenchymal transition(EMT)was analyzed.Results GPM6A was highly expressed in CRC but low or no expression in adjacent normal tissues(P<0.001).The expression of GPM6A increased with the increase of tumor stage(F=2.87,P=0.036).Compared with CRC primary and metastatic lesions,the expression of GPM6A was the highest in CTCs.Among the genes related to EMT status in CRC cells,GPM6A was negatively correlated with galactosidase beta 1 like 2(GLB1L2)(r=-0.13,P<0.05),and positively correlated with collagen typeⅣalpha 2 chain(COL4A2),complement C1s(C1S),collagen type I alpha 2 chain(COL1A2)and caldesmon 1(CALD1)(r=0.26,0.43,0.21,0.67,all P<0.05).Conclusion GPM6A might be a tumor-promoting factor driving the progression of CRC,and a key factor in EMT of CRC cells,and could be a biomarker for monitoring the recurrence of CRC.

关 键 词：结直肠癌 糖蛋白M6A 循环肿瘤细胞 上皮-间质转变 

分 类 号：R735.34[医药卫生—肿瘤]