非小细胞肺癌患者血清UCHL1和HIF-1α表达水平及其与预后的相关性  

Expression of serum UCHL1 and HIF-1α in patients with non-small cell lung cancer and their correlation with prognosis

作　　者：徐亚来[1] 邵润霞[1] 张长玉 XU Ya-lai;SHAO Run-xia;ZHANG Chang-yu(Department of Respiratory and Critical Care Medicine,Second Affiliated Hospital of Zhengzhou University,Zhengzhou,Henan 450014,China)

机构地区：[1]郑州大学第二附属医院呼吸与危重症医学科,河南郑州450014

出　　处：《热带医学杂志》2022年第3期368-372,386,共6页Journal of Tropical Medicine

基　　金：河南省高等学校重点科研项目(18A320062)

摘　　要：目的检测非小细胞肺癌(NSCLC)患者血清泛素羧基末端水解酶L1(UCHL1)、缺氧诱导因子-1α(HIF-1α)的表达,并分析其与患者预后的相关性。方法选取郑州大学第二附属医院2015年1月-2016年6月收治的112例NSCLC患者为研究对象,同期选取在本院进行健康体检且确认未发生肺部癌变者120人为对照组。酶联免疫吸附法(ELISA)检测所有受试者血清UCHL1、HIF-1α表达水平;以NSCLC患者血清UCHL1、HIF-1α表达平均值将其分为UCHL1高表达组(n=58)和UCHL1低表达组(n=54),以及HIF-1α高表达组(n=60)和HIF-1α低表达组(n=52)。对所有NSCLC患者出院后进行随访,随访时间为60个月;Pearson法分析NSCLC患者血清UCHL1、HIF-1α的相关性;Kaplan-Meier生存曲线分析UCHL1、HIF-1α与NSCLC患者预后之间的关系;COX回归分析影响NSCLC患者不良预后的危险因素。结果与对照组比较,NSCLC组患者血清UCHL1、HIF-1α表达显著升高,差异有统计学意义(P<0.05)。NSCLC患者血清UCHL1、HIF-1α表达与患者TNM分期、淋巴结转移有关(P<0.05);NSCLC患者血清UCHL1与HIF-1α表达呈正相关(r=0.423,P<0.05)。NSCLC患者5年内生存率为39.3%,UCHL1高表达组患者5年内生存率(22.4%)低于UCHL1低表达组(57.4%);HIF-1α高表达组5年内生存率(25.0%)低于HIF-1α低表达组(55.8%);差异均有统计学意义(P<0.05)。多因素COX分析表明,淋巴结转移、UCHL1高表达、HIF-1α高表达均是NSCLC患者预后的危险因素(P<0.05)。结论NSCLC患者血清UCHL1、HIF-1α表达与患者临床病理特征及预后有关,可能是NSCLC患者预后的潜在生物标志物。Objective To detect the expression of ubiquitin carboxyl terminal hydrolase L1(UCHL1)and hypoxia inducible factor-1α(HIF-1α)in serum of patients with non-small cell lung cancer(NSCLC),and analyze their correlation with prognosis.Methods A total of 112 patients with NSCLC admitted to the Second Affiliated Hospital of Zhengzhou University from January 2015 to June 2016 were selected as the research objects(NSCLC gorup),and 120 cases of healthy physical examination in our hospital confirmed without lung cancer were selected as the control group;enzyme linked immunosorbent assay(ELISA)was used to detect the expression levels of UCHL1 and HIF-1αin serum;the mean values of serum UCHL1 and HIF-1αin NSCLC patients were divided into UCHL1 high expression group(n=58)and UCHL1 low expression group(n=54)of NSCLC patients,as well as HIF-1αhigh expression group(n=60)and HIF-1αlow expression group(n=52).All NSCLC patients were followed up for 60 months;Pearson method was used to analyze the correlation between serum UCHL1 and HIF-1αin NSCLC patients;Kaplan-Meier survival curve was used to analyze the relationship between UCHL1,HIF-1αand prognosis of NSCLC patients;COX regression analysis was used to analyze the risk factors of poor prognosis in NSCLC patients.Results Compared with that in the control group,the expression of UCHL1 and HIF-1αlevels in serum of NSCLC group patients were significantly higher(P<0.05);the expression levels of UCHL1 and HIF-1αwere correlated with TNM stage and lymph node metastasis in NSCLC patients(P<0.05);the expression levels of UCHL1and HIF-1αwere positively correlated in NSCLC patients(r=0.423,P<0.05).The 5-year survival rate of NSCLC patients was 39.3%;the 5-year survival rate of patients in UCHL1 high expression group(22.4%)was lower than that of patients in UCHL1 low expression group(57.4%);the 5-year survival rate of HIF-1αhigh expression group(25.0%)was lower than that of HIF-1αlow expression group(55.8%);and the differences were statistically significant(P<0.05).Multivariate CO

关 键 词：非小细胞肺癌 泛素羧基末端水解酶L1 缺氧诱导因子-1Α 预后 

分 类 号：R734.2[医药卫生—肿瘤]