白藜芦醇通过MyD88/TLR4/NF-kB信号通路诱导结肠癌细胞凋亡的机制  

Mechanism of apoptosis of colon cancer cells induced by resveratrol through MYD88/TLR4/NF-KB signaling pathway

作　　者：乔璐 杨艳 唐代诗 杜婷 顾勇[3] QIAO Lu;YANG Yan;TANG Daishi;DU Ting;GU Yong(Department of Gastroenterology,the Second Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710004,China;Digestive System Department,the First Affiliated Hospital of AFMU,Xi’an 710032,China;Digestive System Department,Shaanxi Provincial Crops Hospital of Chinese People’s Armed Police Force,Xi’an 710054,China)

机构地区：[1]西安交通大学第二附属医院消化内科,710004 [2]陕西西安大学第一附属医院消化科,710032 [3]武警陕西总队医院消化科,西安710054

出　　处：《武警医学》2022年第4期298-302,共5页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的探讨白藜芦醇通过MyD88/TLR4/NF-kB信号通路影响结肠癌细胞凋亡的机制。方法选取不同剂量白藜芦醇处理24 h后的结肠癌细胞,TUNEL法检测细胞凋亡率的变化,利用RT-PCR和Western blot检测白藜芦醇处理后结肠癌细胞MyD88/TLR4/NF-kB信号通路中关键信号分子的表达变化。结果中高剂量白藜芦醇处理结肠癌细胞系HT-29、sw480和LoVo后,细胞凋亡率显著上升;Bcl-2蛋白和mRNA含量表达下降,Bax、cleaved-Caspase 3蛋白和mRNA含量表达显著升高;细胞上清中VEGF蛋白表达明显降低;细胞中MyD88、TLR4、NF-kB和mRNA含量均明显降低。结论白藜芦醇可能通过MyD88/TLR4/NF-kB信号通路促进结肠癌细胞凋亡从而抑制肿瘤发生发展。Objective To investigate the mechanism of apoptosis of colon cancer cells induced by resveratrol through MyD88/TLR4/NF-KB signaling pathway.Methods With different doses of resveratrol for 24 h,TUNEL assay was used to detect the apoptosis rate of colon cancer cells and Western blot and RT-PCR were employed to test the expression of key signal molecules in MyD88/TLR4/NF-kB signaling pathway.Results The apoptosis rates of HT-29,SW480 and LoVo significantly increased after treatment.The protein and mRNA levels of Bax and Cleaved Caspase 3 significantly increased and Bcl-2 was downregulated.The expression of VEGF protein in cell supernatant decreased significantly.MyD88,TLR4 and NF-KB were significantly down regulated(P<0.05).Conclusions Resveratrol may promote the apoptosis of colon cancer cells through MyD88/TLR4/NF-KB signaling pathway to inhibit the development of tumor.

关 键 词：白藜芦醇 结肠癌 MYD88 TLR4 NF-KB 

分 类 号：R285[医药卫生—中药学]