衰老微环境对非肌层浸润性膀胱癌预后的影响及机制初步探讨  

Influence of aged microenvironment upon the prognosis of non-muscle-invasive bladder cancer and preliminary research on its mechanism

作　　者：王尚任 张爱巧 黄诗桥 王波[1] 马勇[1] 杨永姣 刘晓强 张鲁东[1] 王站成[1] WANG Shangren;ZHANG Aiqiao;HUANG Shiqiao;WANG Bo;MA Yong;YANG Yongjiao;LIU Xiaoqiang;ZHANG Ludong;WANG Zhancheng(Department of Urology,Shanxian Central Hospital,Heze,Shandong,274300,China;Departmentof Urology,Tianjin Medical University General Hospital)

机构地区：[1]单县中心医院济宁医学院附属湖西医院泌尿外科,山东菏泽274300 [2]天津医科大学总医院泌尿外科

出　　处：《临床泌尿外科杂志》2020年第3期196-200,共5页Journal of Clinical Urology

基　　金：山东省医药卫生科技发展计划项目(No:2017ws106).

摘　　要：目的:探讨衰老微环境对非肌层浸润性膀胱癌(NMIBC)预后的影响并初步探讨相关机制。方法:选取单县中心医院初诊的NMIBC患者81例,按照患者年龄分为低龄组(<65岁)和高龄组(≥65岁)两组,比较分析两组患者无复发生存期及无进展生存期,采用Cox回归模型分析影响NMIBC患者预后的危险因素。并留取同期癌旁正常膀胱黏膜组织30例作为对照,采用免疫组化法检测分析正常膀胱黏膜、低龄组及高龄组NMIBC中分泌型Frizzled相关蛋白2(secreted frizzled-related proteins 2,sFRP2)及β-catenin的表达水平。结果:低龄组患者的无复发生存率和无进展生存率均高于高龄组患者(P<0.05),多因素分析发现年龄是NMIBC患者复发和进展的独立危险因素。sFRP2在低龄组及高龄组NMIBC组织中,表达的阳性率均低于正常膀胱黏膜(P<0.05);高龄组sFRP2的表达阳性率明显高于低龄组(P<0.05)。β-catenin低龄组及高龄组NMIBC组织中表达的阳性率均高于正常膀胱黏膜组织(P<0.05);高龄组β-catenin的表达阳性率明显低于低龄组(P<0.05)。结论:衰老微环境可能在膀胱癌的发生发展中发挥了一定作用。sFRP2及β-catenin可能参与了衰老微环境对膀胱癌发生发展过程的调控。Objective:To investigate the prognosis of human non-muscle-invasive bladder cancer in aged microenvironment,and investigate the related molecular mechanisms.Method:According to the age,81 patients of non-muscle-invasive bladder cancer were divided into young group(<65 years old)and aged group(≥65 years old).The recurrence-free survival(RFS)and progression-free survival(PFS)of two groups were analyzed.Risk factors influencing prognosis of non-muscle-invasive bladder cancer were analyzed by using the Cox proportional hazards regression model.The expressions of sFRP2 andβ-catenin were detected by immunohistochemical method in normal bladder tissues,young and aged groups of non-muscle-invasive bladder cancer tissues.Result:The recurrence and progression rates in the aged group is higher than those in the young group,and the RFS and PFS were shorter(P<0.05).In the multivariable Cox analysis,we found that age was an independent predictor of RFS and PFS.Compared with normal bladder tissue,sFRP2 was lowly expressed in both young and aged groups of non-muscle-invasive bladder cancer tissues(P<0.05).The level of sFRP2 was significantly higher in the aged group than that in the young group(P<0.05).Higher expression ofβ-catenin was found in both young and aged groups of non-muscle-invasive bladder cancer tissues compared with the normal bladder tissue(P<0.05).The level ofβ-catenin was significantly lower in the aged group than that in the young group(P<0.05).Conclusion:The aged microenvironment is an important factor influencing the survival rate of the patients with bladder cancer.In aged microenvironment,sFRP2 andβ-catenin are closely correlated with the carcinogenesis and progression of non-muscle-invasive bladder cancer in the aged microenvironment.

关 键 词：衰老微环境 非肌层浸润性膀胱癌 SFRP2 Β-CATENIN 预后 

分 类 号：R737.14[医药卫生—肿瘤]