Simultaneous Screening and Analysis of Anti-inflammatory and Antiproliferative Compounds from Euphorbia maculata Combining Bio-affinity Ultrafiltration with Multiple Drug Targets  

作　　者：Tojofaniry Fabien Rakotondrabe Min-Xia Fan Yong-Li Zhang Ming-Quan Guo 

机构地区：[1]CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture,Wuhan Botanical Garden,Chinese Academy of Sciences,Wuhan 430074,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]Sino-Africa Joint Research Center,Chinese Academy of Sciences,Wuhan 430074,China [4]Innovation Academy for Drug Discovery and Development,Chinese Academy of Sciences,Shanghai 201203,China

出　　处：《Journal of Analysis and Testing》2022年第2期98-110,共13页分析检测（英文）

摘　　要：Euphorbia maculata has long been used for managing different impairments in Asian countries.However,its antioxidant,anti-inflammatory and antiproliferative potentialities,along with its potential bioactive compounds remain unexplored.In this context,a bio-a ffinity ultrafiltration strategy was developed to fish out ligand candidates against Cycloxygenase-2(COX-2),Topoisomerase I(Topo I),and TopoisomeraseⅡ(TopoⅡ).Thereafter,lead compounds activities were assessed in silico and in vitro for ascertaining the screening results and forecasting its corresponding activities.As a result,the E.maculata ethyl acetate(EMEA)fraction showed interesting COX-2 inhibition activity with an IC 50 value of 0.67±0.09μg/mL,as well as good growth inhibitions for three malignant cell lines.EMEA chemical fingerprinting was also conducted to enable a tentative identification of 17 compounds,among which,11 were assessed as ligand candidates to COX-2,8 compounds to Topo I,and 10 compounds to TopoⅡ.Dihydromyricetin and quercetin-3-O-arabinoside were revealed to be multipotent compounds which exerted good a ffinity to the three targeted enzymes,and also supported with their molecular docking simulations and in vitro assay validations.The interrelationship between E.maculata’s associated activities(antioxidant,anti-inflammatory and antiproliferative)was revealed with the corresponding multipotent phytochemical active components from this work.It also provided a useful direction for its empirical traditional use and further explorations in the near future.

关 键 词：Euphorbia maculata Antioxidant Anti-infl ammatory ANTIPROLIFERATIVE UF-UPLC-QTOF-MS/MS Multipotent compounds 

分 类 号：R284.1[医药卫生—中药学]