微波强化纳米金杂化CRL脂肪酶催化甾醇油酸酯的合成  

Optimization of Microwave Enhanced Gold Nanoparticles-CRL Hybridase (AuNPs-CRL) Catalyzed Synthesis of Phytosterol Esters

作　　者：尹一 孙立瑞 方祁利 夏春谷[2] 辛嘉英[1,2] YIN Yi;SUN Li-rui;FANG Qi-li;XIA Chun-gu;XIN Jia-ying(Key Laboratory for Food Science and Engineering,Harbin Uninversity of Commerce,Harbin150076,China;State Key Laboratory for Oxo Synthesis&Selective Oxidation,Lanzhou Institute of Chemical Physics,Chinese Academy of Science,Lanzhou730000,China)

机构地区：[1]哈尔滨商业大学食品科学与工程重点实验室,黑龙江哈尔滨150076 [2]中国科学院兰州物理化学研究所,羰基合成与选择氧化国家重点实验室,甘肃兰州730000

出　　处：《分子催化》2022年第6期522-533,共12页Journal of Molecular Catalysis(China)

基　　金：中央支持地方高校改革发展资金人才培养支持计划项目(304017)

摘　　要：为进一步提高植物甾醇的脂溶性、降低熔点,提出了一种在微波辐射条件下、利用纳米金杂化CRL脂肪酶为催化剂的植物甾醇油酸酯的合成方法.以植物甾醇的转化率为指标,通过响应面法确定合成植物甾醇酯的最佳工艺条件,并对最优条件进行了验证.微波强化纳米金杂化CRL脂肪酶催化植物甾醇油酸酯的最优条件为:AuNPs粒径为15 nm、AuNPs-CRL杂化酶的添加量为8%、微波功率为320 W、反应时间为64 min,在此优化条件下测得植物甾醇的转化率为91.24%±0.42%,收率为83.73%,与预测值吻合度良好.In order to further improve the lipid solubility and lower the melting point of phytosterols,an efficient method for the synthesis of phytosterol oleate under microwave irradiation using AuNPs-CRL hybridase as a catalyst was proposed.The process conditions for the synthesis of phytosterol esters were optimized by response surface analysis with the conversion rate of phytosterols as indicator,and the optimal conditions were verified.The optimization conditions for the microwave-enhanced AuNPs-CRL hybridas catalyzed the synthesis of phytosterol oleate were:the particle size of AuNPs was 15 nm,the addition of AuNPs-CRL hybridas was 8%,the microwave power was 320 W,and the reaction time was 64 min.The conversion rate of phytosterol was 91.24%±0.42%,phytosterol ester yield was 83.73%,which was in good agreement with the predicted value.

关 键 词：植物甾醇酯 纳米金 杂化酶 微波 

分 类 号：TQ225.24[化学工程—有机化工]