分散固相萃取-液相色谱法测定食用槟榔中苯并(a)芘含量  

Determination of benzo(a)pyrene in edible areca-nut by dispersive solid-phase extraction coupled with liquid chromatography

作　　者：余辅松 邓华 梁振纲 邓世明[3] YU Fu-song;DENG Hua;LIANG Zhen-gang;DENG Shi-ming(Haikou Branch of Hainan Institute for Food and Drug Control,Haikou 570300,China;Hainan Simcere Co.,Ltd.,Haikou 570216,China;College of Marine Science,Hainan University,Haikou 570228,China;Technology Center of Hainan Entry-Exit Inspection and Quarantine Bureau,Haikou 570311,China)

机构地区：[1]海南省食品药品检验所海口分所,海口570300 [2]海南先声药业有限公司,海口570216 [3]海南大学海洋学院,海口570228 [4]海南出入境检验检疫局技术中心,海口570311

出　　处：《中华中医药杂志》2019年第12期5930-5932,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家质量监督检验检疫总局科技计划项目(No.2017IK146).

摘　　要：目的:建立了分散固相萃取-高效液相色谱检测食用槟榔中苯并(a)芘含量的方法。方法:色谱柱采用安捷伦ZORBAX SB-C18(250mm×4.6mm,5μm),流动相为乙腈-水(88∶12);流速为1.2mL/min,荧光检测波长:Ex:364nm,Em:404nm。结果:苯并(a)芘在2.0~20.0ng/mL范围内呈良好线性关系,r=0.9997;方法检测限为1.0μg/kg。本品平均回收率为99.47%,RSD(n=6)为1.274%。结论:该法快速、简便、准确,适用于食用槟榔中苯并(a)芘的检测。Objective:To develop a dispersive solid-phase extraction-liquid chromatography method for the analysis of benzo(a)pyrene in edible areca-nut.Methods:Agilent ZORBAX SB-C18(250 mm×4.6 mm,5μm)chromatographic column was adopted.The mobile phase was acetonitrile-water(88∶12),and the rate was 1.2 mL/min.Fluorescence detection wavelength was Ex:364 nm,Em:404 nm.Results:Benzo(a)pyrene showed a perfect linearity in the range of 2.0-20.0 ng/mL(r=0.9997),and the limit of detection(LOD)for this method was 1.0μg/kg.The average recovery rate of this product was 99.47%and RSD(n=6)was 1.274%.Conclusion:The method is fast,convenient and accurate,and is applicable for the analysis of benzo(a)pyrene in the edible areca-nut.

关 键 词：食用槟榔 分散固相萃取 苯并(A)芘 含量 

分 类 号：R284.1[医药卫生—中药学]