水牛ApoB基因多态性与泌乳性状关联分析  

Association Analysis Between ApoB Polymorphisms and Milk Production Traits in Buffalo(Bubalus bubalis)

作　　者：林雨昕 曹家瑄 孙晖 李俊 李华钊 吴祺鹏 邓廷贤[4] 杨利国[1] 滑国华[1,2,5] Lin Yuxin;Cao Jiaxuan;Sun Hui;Li Jun;Li Huazhao;Wu Qipeng;Deng Tingxian;Yang Liguo;Hua Guohua(Key Laboratory of Ministry of Education for Agricultural Animal Genetics,Breeding and Reproduction,College of Animal Science and Technology,Huazhong Agricultural University,Wuhan,430070;Shenzhen Institute of Nutrition and Health,Huazhong Agricultural University,Shenzhen Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Genome Analysis Laboratory of the Ministry of Agriculture,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,518000;Key Laboratory of Immunology,College of Basic Medicine,Zunyi Medical University,Zunyi,563000;Guangxi Key Laboratory of Buffalo Genetice,Breeding and Reproduxtion,Guangxi Buffalo Research Institute,Chinese Academy of Agricultural Sciences,Nanning,530000;National Center for International Research on Animal Genetics,Breeding and Reproduction,Key Laboratory of Smart Farming for Agricultural Animals,Wuhan,430070)

机构地区：[1]华中农业大学动物科技学院,农业动物遗传育种与繁殖教育部重点实验室,武汉430070 [2]华中农业大学深圳营养与健康研究院,中国农业科学院深圳农业基因组研究所和岭南现代农业科学与技术广东省实验室深圳分中心,深圳518000 [3]遵义医科大学基础医学院免疫教研室,遵义563000 [4]中国农业科学院广西水牛研究所,广西水牛遗传繁育重点实验室,南宁530000 [5]国家动物遗传育种与繁殖国际联合研究中心,农业农村部智慧养殖技术重点实验室,武汉430070

出　　处：《基因组学与应用生物学》2022年第6期1180-1192,共13页Genomics and Applied Biology

基　　金：中央高校基本科研业务费专项资金(2662022DKYJ002);国家现代农业产业技术体系专项基金(CARS36);华中农业大学-中国农业科学院深圳农业基因组研究所合作基金(SZYJY2022026)共同资助

摘　　要：本研究旨在探究水牛(Bubalus bubalis)载脂蛋白B基因(ApoB)在地中海水牛群体中的遗传多态性,并与地中海水牛泌乳性状进行关联分析,筛选水牛产奶性状分子标记。本研究以350头地中海纯种奶水牛为研究对象,利用直接测序法和基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)法筛选基因多态性(single nucleotide polymorphisms,SNPs)与基因分型,并进一步分析单倍型与泌乳性状的相关性。结果显示,在地中海水牛群体ApoB基因中筛选到10个SNPs,分别位于启动子(g.-1718A>G、g.-1823G>C和g.-2063A>C)、第7内含子(g.8105G>A)、第26内含子(g.30643G>A)、第5外显子(g.5903G>A)、第26外显子(g.30879T>C、g.33638A>C和g.37375G>A)和第29外显子(g.40787A>G)区域。10个SNPs位点多态信息含量均大于0.25且小于0.5,属于中度多态。其中g.-1718A>G、g.-1823G>C、g.-2063A>C、g.37375G>A和g.40787A>G突变位点与地中海水牛乳蛋白率显著相关,g.5903G>A和g.40787A>G位点与地中海水牛乳脂率显著相关,g.37375G>A与地中海水牛270天乳蛋白量和乳脂量显著相关。单倍型Block1(g.-1718A>G、g.-1823G>C和g.-2063A>C)与270天乳蛋白量和乳蛋白率显著相关;单倍型Block2(g.30643G>A和g.33638A>C)与270天乳脂含量、乳脂率、270天乳蛋白量以及乳蛋白率显著相关;单倍型Block3(g.37375G>A和g.40787A>G)与乳蛋白率显著相关;g.33638A>C、g.37375G>A、g.40787A>G以及单倍型Block2和Block3突变型蛋白质理化性质和二级结构发生改变。本研究共筛选到6个SNPs和3个单倍型Block与水牛泌乳性状显著相关,其中单倍型Block2的H3H3为最优单倍型,可作为水牛产奶性状潜在分子标记。This study aims to identify ApoB polymorphisms in buffalo(Bubalus bubalis),and to further explore the molecular markers associated with buffalo lactation traits.In the present study,ApoB single nucleotide polymorphisms(SNPs)were identified by DNA sequencing in a total of 350 Mediterranean buffaloes,and MALDITOF-MS was employed for genotyping.Association analysis between ApoB haplotype and milk production traits was carried by using SAS9.4 software.Besides,the physical,chemical properties and structure of ApoB protein were predicted by using bioinformatic tools.In the present study,10 SNPs were screened,3 of which located in the promoter(g.-1718A>G,g.-1823G>C,and g.-2063A>C),intron 7(g.8105G>A),1 of which located in the intron 26(g.30643G>A),1 of which located in the exon 5(g.5903G>A),3 of which located in the exon 26(g.30879T>C,g.33638A>C,g.37375G>A)and 1 of which located in the exon 29(g.40787A>G).All the identified SNPs were moderately polymorphic(0.25<PIC<0.50).Association analysis showed that g.-1718A>G,g.-1823G>C,g.-2063A>C,g.37375G>A,and g.40787A>G were significantly related to milk protein percentage,g.5903G>A and g.40787A>G were significantly related to milk fat percentage,and g.37375G>A was significantly related to 270-day milk protein and 270-days milk fat yield.Haplotype block1(g.-1718A>G,g.-1823G>C,and g.-2063A>C)was significantly associated with 270-days milk protein yield and milk protein percentage.Haplotype block2(g.30643G>A and g.33638A>C)was significantly related to 270-days milk fat and milk protein yield,milk fat percentage,and milk protein percentage.Haplotype block3(g.37375G>A and g.40787A>G)was significantly related to the milk protein percentage,g.33638A>C,g.37375G>A,g.40787A>G and haplotype block3(g.37375G>A and g.40787A>G)affected the physical and chemical properties and the secondary structure of the ApoB protein.In conclusion,a total of 6 SNPs and 3 haplotype blocks were identified significantly associated with buffalo milk production traits.Among them,H3H3 in haplotype block2 was the

关 键 词：水牛 APOB 泌乳性状 性状关联分析 基因多态性 

分 类 号：S823.83[农业科学—畜牧学]