Effect of Sanwu Huangqin Decoction on IFN-γ and IL-17A levels in mice with Behcet's disease  

作　　者：Yong-Zhu Piao Dan-Nan Sun Yin Shao Yan Zhang Bing-Yu Wang 

机构地区：[1]The First Affiliated Hospital of Heilongjiang University of traditional Chinese medicine,Heilongjiang,Harbin,150040 [2]The Second Affiliated Hospital of Heilongjiang University of traditional Chinese medicine,Harbin,Heilongjiang,150001 [3]Heilongjiang University of traditional Chinese medicine,Heilongjiang,Harbin,150040 [4]Nangang Branch of Heilongjiang College of traditional Chinese medicine,Harbin,Heilongjiang,1500006

出　　处：《Journal of Hainan Medical University》2020年第7期6-10,共5页海南医学院学报（英文版）

基　　金：General Project of Natural Science Funds of Heilongjiang Province(No.H2017064)

摘　　要：Objective:To investigate the effect of Sanwu Huangqin Decoction on IFN-γand IL-17A levels in mice with Behcet's disease;Methods:Clinical samples of patients with Behcet's disease were collected and mononuclear cells were isolated.High throughput RNA-seq was used to identify the different genes between Behcet's disease and healthy subjects.Mouse model was constructed by shrimp myosin sensitization and treated with Sanwu Huangqin decoction.The levels of IFN-γand IL-17A in peripheral blood and mononuclear cells were detected by ELISA and flow cytometry.The expression level of TBX21 gene in spleen was detected by RT-PCR;Results:Compared with healthy subjects,31 differential genes were identified in BD patients,of which 19 genes were up-regulated and 12 genes were down regulated.PPI analysis showed that the top 5 genes were TBX21,STAT3,IL15,PRF1 and IFNG.The results of animal experiments showed that the levels of IFN-γand IL-17A in the peripheral blood and PBMC of the model group were significantly higher compared with the control group(P<0.01).Sanwu Huangqin decoction could significantly reduce the levels of IFN-γand IL-17A in the peripheral blood and PBMC of mice(P<0.05 or P<0.01).RT-PCR results showed that the expression of TBX21 mRNA in spleen tissue of model group was significantly higher than that of the control group(P<0.01).Sanwu Huangqin decoction could significantly down-regulate the expression of TBX21 mRNA in spleen(P<0.05 or P<0.01);Conclusion:Sanwu Huangqin decoction reduce the levels of IFN-γand IL-17A by inhibiting the expression of TBX21 mRNA,so as to achieve the effect of treating BD.

关 键 词：Sanwu Huangqin decoction Behcet's disease Prawn protomyosin TBX21 gene 

分 类 号：R73[医药卫生—肿瘤]