Promoting wound healing effect of the extract of callicarpa nudiflora and preparation technology of the external ointment  

作　　者：Ying Liu Jun-Qing Zhang Wei-Yong Lai 

机构地区：[1]Institute of Pharmacy,Hainan Medical University,Research and Development Key Laboratory of Tropical Medicinal Plants of HaiNan Province

出　　处：《Journal of Hainan Medical University》2019年第17期45-49,共5页海南医学院学报（英文版）

基　　金：No: 2018CXTD339, Hainan Natural Science Foundation Innovation Research Team Project, Research on three external preparations of Callicarpa nudiflora.

摘　　要：Objective: To study the effect of the extracts from Callicarpa nudiflora on wound healing and the preparation of ointment. Methods: Totally 50 KM mice were randomly divided into five groups, which were negative control group ,positive control group, high, middle and low dose groups of Callicarpa nudiflora ointment. Mice ear swelling test was conducted to investigate the effect of Callicarpa nudiflora induced by xylene, Bacteriostasis experiments were conducted to observe the bacteriostasis effects of Callicarpa nudiflora on five indicator bacteria, i.e. Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Bacillus subtilis, and Staphylococcus aureus in vitro. A total of 30 new zealand white rabbit with half male and half female were randomly divided into five groups, which were negative control group, positive control group, high, middle and low groups of Callicarpa nudiflora ointment. The rabbits were treated with sterilizing blade and treated with external medicine to observe the effect of the extracts from Callicarpa nudiflora on wound healing. Orthogonal test was used to determine the preparation method of Callicarpa nudiflora ointment,Physical properties, centrifugal stratification test, heat and cold resistance test were conducted. Results:Bacteriostasis experiments in vitro showed high dose group of Callicarpa nudiflora extracts got 16.8 cm antimicrobial zone of Pseudomonas aeruginosa. The experimental results showed that the ear swelling degree of mice induced by xylene was significantly reduced in the middle and high dose groups. All the treatment groups could promote skin healing of rabbit significantly compared with negative control group. There were significantly differences between all the treatment groups and negative control group on the wound healing rate. The optimum preparation process was determined by orthogonal screening test. The optimal formula was as follows: Twain-801.2 g, glycerin monostearate 4.7 g, glycerinum 0.9 g, liquid paraffin 10.8 g, Span801.4 g,solid paraffin 1.4 g,vaseli

关 键 词：EXTRACT of CALLICARPA nudiflora Anti-inflammatory EXTERNAL OINTMENT In VITRO ANTISEPTIC 

分 类 号：R28[医药卫生—中药学]