Isolation and characterization of the GbVIP1 gene and response to Verticillium wilt in cotton and tobacco  

作　　者：ZHANG Kai ZHAO Pei WANG Hongmei ZHAO Yunlei CHEN Wei GONG Haiyan SANG Xiaohui CUI Yanli 

机构地区：[1]Institute of Cotton Research of Chinese Academy of Agricultural Sciences/State Key Laboratory of Cotton Biology,Anyang 455000,Henan,China

出　　处：《Journal of Cotton Research》2019年第4期18-28,共11页棉花研究（英文）

摘　　要：Background:Verticillium wilt is a serious soil-borne vascular disease that causes major losses to upland cotton (Gossypium hirutum L.) worldwidely every year.The protein VIP1 (VirE2 interaction protein 1),a bZIP transcription factor,is involved in plant response to many stress conditions,especially pathogenic bacteria.However,its roles in cotton response to Verticillium wilt are poorly understood.Results:The GbVlP1 gene was cloned from resistant sea-island cotton (G.barbadense) cv.Hai 7124.Expression of GbVIP1 was up-regulated by inoculation with Verticillium dahliae and exogenous treatment with ethylene.Results of virus-induced gene silencing suggested that silencing of GbVIP1 weakened cotton resistance to Verticillium wilt.The heterologous expression of GbVIP1 in tobacco showed enhanced resistance to Verticillium wilt.The PR1,PR1-like and HSP70 genes were up-regulated in GbVIP1 transgenic tobacco after Verticillium wilt infection.Conclusion:Our results suggested that GbVlP1 increased plant resistance to Verticillium wilt through up-regulating expressions of PR1,PR1-like,and HSP70.These results provide new approaches to improving resistance to Verticillium wilt in upland cotton and also have great potential for disease-resistance breeding of cotton.

关 键 词：COTTON VIP1 VERTICILLIUM WILT 

分 类 号：R73[医药卫生—肿瘤]