Characterization and frequency of antibiotic resistance related to membrane porin and efflux pump genes among Acinetobacter baumannii strains obtained from burn patients in Tehran, Iran  

作　　者：Majid Noori Behzad Mohsenzadeh Aghil Bahramian Fatemeh Shahi Habibollah Mirzaei Saeed Khoshnood 

机构地区：[1]Department of Infectious Diseases,Faculty of Medicine,Army University of Medical Sciences,Tehran,Iran [2]Golestan Hospital,Army University of Medical Sciences,Tehran,Iran [3]Department of Microbiology,school of Medicine,Shahid Beheshti University of Medical sciences,Tehran,Iran [4]Department of Microbiology,Faculty of Medicine,Ahvaz Jundishapur University of Medical Sciences,Ahvaz,Iran [5]Student Research Committee,Ahvaz Jundishapur University of Medical Sciences,Ahvaz,Iran [6]Hepatitis Research Center,Lorestan University of Medical Sciences,Khorramabad,IR,Iran

出　　处：《Journal of Acute Disease》2019年第2期63-66,共4页急性病杂志（英文版）

摘　　要：Objective: To explore the characterization and frequency of antibiotic resistance related to membrane porin and efflux pump genes among Acinetobacter baumannii (A. baumannii) strains obtained from burn patients in Tehran, Iran. Methods: In this cross-sectional descriptive study, 100 strains of A. baumannii isolated from burn patients visiting teaching hospitals of Tehran were collected from January 2016 to November 2017. After A. baumannii strains were confirmed, antimicrobial susceptibility testing was done via Kirby-Bauer disc diffusion method according to the Clinical and Laboratory Standards Institute guidelines. PCR amplification was performed for detection of β-lactamase adeR, OprD, adeS genes among A. baumannii strains. Results: All isolates (100%) were resistant to ceftazidime, cefotaxime, cefepime, ciprofloxacin, and piperacillin, and most isolates indicated high resistance (95%-97%) to meropenem, imipenem, gentamicin, ceftriaxone, trimethoprim-sulfamethoxazole, piperacillin-tazobactam, amikacin, and tetracycline. The most effective antibiotic against A. baumannii isolates was colistin (97% sensitivity), followed by tigecycline. The frequency of OprD, adeS, and adeR genes were 98%, 91%, and 77%, respectively. Conclusions: This study shows that the majority of A. baumannii isolates are highly resistant to the antibiotics most commonly used in burn patients. Also, high distribution of OprD and adeRS genes may be responsible for the observed resistances among A. baumannii isolates that demonstrate the possible role of both efflux pumps in simultaneous of carbapenemase production during antibiotic resistance.

关 键 词：ACINETOBACTER BAUMANNII MULTIDRUG resistance Burn EFFLUX pump genes 

分 类 号：R73[医药卫生—肿瘤]