Beta2-glycoprotein I cooperate with hepatitisB surface antigen promotes hepatocellular carcinogenesis via the nuclear factor kappa B signalpathway were enhanced by the lipopolysaccharide  

作　　者：Xue-Li Ding Xue Jing Nai-Jun Han Zi-Bin Tian Pu-Jun Gao Lin Yang Ya-Nan Yu 

机构地区：[1]Department of Gastroenterology,the Affiliated Hospital of Qingdao University,Qingdao 266003,Shandong,China [2]Department of Research and development,Yebio Bioengineering Co.,Ltd.of Qingdao,Qingdao 266114,Shandong,China [3]Department of Hepatology,the First Hospital of Jilin University,Changchun 310021,Jilin,China

出　　处：《Hepatoma Research》2018年第4期25-32,共8页肝癌研究（英文版）

摘　　要：Aim: We aimed to elucidate whether beta2-glycoprotein I (β2GPI) cooperation with hepatitis B surface antigen (HBsAg) promoted hepatocellular carcinogenesis enhanced by the lipopolysaccharide (LPS) via activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and expression of tumor necrosis factor-α(TNF-α), interleukin-1β (IL-1β), and alpha fetal protein (AFP) in liver cancer cells. Methods: Liver cancer cells (SMMC-7721) were transiently transfected with β2GPI and/or HBsAg and were subjected to LPS treatment. TNF-α, IL-1β, and AFP expression were measured in all groups by ELISA. NF-κB activation was assessed by non-radioactive electrophoretic mobility shift assay (EMSA) and was quantified in all groups. Results: Cells transfected with β2GPI and/or HBsAg induced activation of NF-κB, with the highest activation seen in the doubly β2GPI- and HBsAg-transfected cells treated with LPS. Non-transfected cells treated with LPS exhibited lower activation compared to either β2GPI- or HBsAg-transfected cells with LPS treatment. In addition, cells transfected with β2GPI and/or HBsAg induced significantly increased expression of TNF-α, IL-1β and AFP, with the highest levels again seen in the doubly β2GPI- and HBsAg-transfected cells treated with LPS. Conclusion: These observations suggest that the activity of NF-κB induced by β2GPI and HBsAg was enhanced by LPS. Expression of TNF-α, IL-1β and AFP increased in β2GPI and HBsAg cotransfected liver cancer cells.

关 键 词：Beta2-glycoproten I hepatitis B surface antigen LIPOPOLYSACCHARIDE liver cancer SMMC-7721 nuclear factor kappa-light-chain-enhancer of activated B cells 

分 类 号：R73[医药卫生—肿瘤]