Mutations and intron polymorphisms in voltage-gated sodium channel genes of different geographic populations of Culex pipiens pallens/Culex pipiens quinquefasciatus in China  

作　　者：Wenyu Li Delong Ma Qunzheng Mu Xinxin Zhou Dongdong Hua Chunchun Zhao Qiyong Liu Jun Wang Fengxia Meng 

机构地区：[1]State Key Laboratory of Infectious Disease Prevention and Control,Department of Vector Biology and Control,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,WHO Collaborating Centre for Vector Surveillance and Management,Beijing 102206,People’s Republic of China [2]Ningxia Center for Disease Prevention and Control,YinChuan 750004,NingXia,People’s Republic of China [3]Jinan Shizhong Center for Disease Control and Prevention,Jinan 250000,Shandong,China [4]Vanke School of Public Health,Tsinghua University,Beijing,People’s Republic of China [5]Weifang No.2 People’s Hospital,Weifang 261000,Shandong,People’s Republic of China [6]Beijing Daxing Center for Disease Control and Prevention,Beijing 102600,People’s Republic of China [7]Jinan Second Maternal and Child Health Hospital,Jinan 250000,Shandong,China

出　　处：《Infectious Diseases of Poverty》2024年第2期6-16,共11页贫困所致传染病（英文）

基　　金：funded by the National Science and Technology Major Project of China(No.2018ZX10101002-002)

摘　　要：Background Culex pipiens pallens and Culex pipiens quinquefasciatus are the dominant species of Culex mosquitoes in China and important disease vectors.Long-term use of insecticides can cause mutations in the voltage-gated sodium channel(vgsc)gene of mosquitoes,but little is known about the current status and evolutionary origins of vgsc gene in different geographic populations.Therefore,this study aimed to determine the current status of vgsc genes in Cx.p.pallens and Cx.p.quinquefasciatus in China and to investigate the evolutionary inheritance of neighboring downstream introns of the vgsc gene to determine the impact of insecticides on long-term evolution.Methods Sampling was conducted from July to September 2021 in representative habitats of 22 provincial-level administrative divisions in China.Genomic DNA was extracted from 1308 mosquitoes,the IIS6 fragment of the vgsc gene on the nerve cell membrane was amplified using polymerase chain reaction,and the sequence was used to evaluate allele frequency and knockdown resistance(kdr)frequency.MEGA 11 was used to construct neighborjoining(NJ)tree.PopART was used to build a TCS network.Results There were 6 alleles and 6 genotypes at the L1014 locus,which included the wild-type alleles TTA/L and CTA/L and the mutant alleles TTT/F,TTC/F,TCT/S and TCA/S.The geographic populations with a kdr frequency less than 20.00%were mainly concentrated in the regions north of 38°N,and the geographic populations with a kdr frequency greater than 80.00%were concentrated in the regions south of 30°N.kdr frequency increased with decreasing latitude.And within the same latitude,the frequency of kdr in large cities is relatively high.Mutations were correlated with the number of introns.The mutant allele TCA/S has only one intron,the mutant allele TTT/F has three introns,and the wild-type allele TTA/L has 17 introns.Conclusions Cx.p.pallens and Cx.p.quinquefasciatus have developed resistance to insecticides in most regions of China.The neighboring downstream introns of the vgsc gene g

关 键 词：Culex pipiens pallens Culex pipiens quinquefasciatus vgsc gene MUTATION Frequency INTRON HAPLOTYPE China 

分 类 号：R384.1[医药卫生—医学寄生虫学]