An accurate diagnostic approach for urothelial carcinomas based on novel dual methylated DNA markers in small-volume urine  

作　　者：Yucai Wu Di Cai Jian Fan Chang Meng Shiming He Zhihua Li Lianghao Zhang Kunlin Yang Aixiang Wang Xinfei Li Yicong Du Shengwei Xiong Mancheng Xia Tingting Li Lanlan Dong YanqingGong Liqun Zhou Xuesong Li 

机构地区：[1]Department of Urology,Peking University First Hospital,Beijing 100034,China [2]Institute of Urology,Peking University,Beijing 100034,China [3]National Urological Cancer Center,Beijing 100034,China [4]Urogenital Diseases(Male)Molecular Diagnosis and Treatment Center,Peking University,Beijing 100034,China [5]Wuhan Ammunition Life-tech Company,Ltd.,Wuhan,Hubei 430200,China

出　　处：《Chinese Medical Journal》2024年第2期232-234,共3页中华医学杂志（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China(Nos.82273350,81972380,81872083,and 81772703);the Natural Science Foundation of Beijing(No.7222190);the Beijing Municipal Science&Technology Commission Project(No.Z201100005420029);National High Level Hospital Clinical Research Funding(Scientific Research Seed Fund of Peking University First Hospital,No.2022SF18)

摘　　要：Diagnostic methods for urothelial carcinomas(UCs)are often invasive or have suboptimal accuracy.Methylation of exfoliated cell DNA or cell-free DNA in urine has shown great promise in the diagnosis of UCs.However,most current studies have focused on bladder cancer(BCa),and only a few high-plex methylated DNA panels based on large-volume urine have been reported to exhibit both high sensitivity and specificity.[1,2]The purpose of this study was to identify universal biomarkers for BCa and upper tract urothelial carcinoma(UTUC)using a small volume of urine.We developed a dual-target diagnostic panel comprising the novel marker AL021918.2 and the well-known BCa biomarker Vimentin(VIM).This panel can accurately detect UCs using only 1.8 mL of full-voided urine.

关 键 词：URINE BLADDER DIAGNOSIS 

分 类 号：R737.1[医药卫生—肿瘤]