基质金属蛋白酶-8对食管鳞癌EC109细胞增殖和迁移的影响  

The effect of matrix metalloproteinase-8 protein on proliferation and migration of squamous cell carcinoma EC109 cells of the esophagus

作　　者：戢得天 文卉 刘晓波 童强 Ji Detian;Wen Hui;Liu Xiaobo;Tong Qiang(Department of Heart Function Examination,Taihe Hospital,Affiliated Hospital of Hubei University of Medicine,Shiyan 442000,China;Department of Gastroenterology,Taihe Hospital,Affiliated Hospital of Hubei University of Medicine,Shiyan 442000,China)

机构地区：[1]十堰市太和医院(湖北医药学院附属医院)心功能室,十堰442000 [2]十堰市太和医院(湖北医药学院附属医院)消化内科,十堰442000

出　　处：《中国医师杂志》2023年第10期1496-1501,共6页Journal of Chinese Physician

基　　金：湖北省卫生健康委科研项目(WJ2021M046、WJ2023Q022);十堰市科技局项目(21Y19)太和医院院级课题(2020JJXM032)

摘　　要：目的明确基质金属蛋白酶-8(MMP-8)对食管鳞癌EC109细胞增殖、迁移能力的影响,探索其可能的作用机制。方法培养食管上皮细胞Het-1A和食管鳞癌细胞株(EC109、CEC2、KYSE150、TE-1细胞),采用PCR实验筛选出MMP-8低表达细胞系EC109细胞进行质粒转染,其中MMP-8过表达质粒为MMP-8过表达组,空载体质粒为空载体组。采用流式细胞分选技术扩增培养成功转染的细胞,并构建稳转细胞系。通过MTT实验和细胞划痕愈合实验检测两组细胞增殖、迁移能力的变化,通过qRT-PCR实验检测两组细胞中增殖迁移相关基因的mRNA表达情况。结果在各食管鳞癌细胞株中,MMP-8在EC109细胞系中表达水平最低。与空载体组相比,MMP-8过表达组细胞增殖、迁移能力明显增强,增殖、迁移相关基因AKT1、RAC1、p53、EGFR、MTA1和RARRES1 mRNA表达水平明显升高,差异有统计学意义(均P<0.05)。结论MMP-8蛋白可增强食管鳞癌EC109细胞的增殖、迁移能力。MMP-8过表达可促进增殖、迁移相关基因mRNA表达,可能与其促进EC109细胞增殖、迁移有关。Objective To investigate the effects of matrix metalloproteinase-8(MMP-8)on the proliferation and migration of esophageal squamous cell carcinoma EC109 cells,and to explore its possible mechanism.Methods Esophageal epithelial cells Het-1A and esophageal squamous cell carcinoma cell lines(EC109,CEC2,KYSE150,TE-1 cells)were cultured.Using polymerase chain reaction(PCR)experiments,MMP-8 low expression cell line EC109 cells were screened for plasmid transfection.The MMP-8 overexpression plasmid was MMP-8 overexpression group,and the empty plasmid was the empty vector group.Flow cytometry was used to screen and amplify the transfected cells,and stable transfected cells were constructed.The changes of proliferation and migration were detected by methyl thiazolyl tetrazolium(MTT)and scratch test,and the mRNA expression of proliferation and migration related genes was detected by quantitative real-time polymerase chain reaction(qRT-PCR).Results MMP-8 expression was lowest in EC109 cells among all esophageal squamous cell carcinoma cell lines.Compared with the empty vector group,the proliferation and migration ability of MMP-8 overexpression group cells were significantly enhanced,and the mRNA expression levels of proliferation and migration related genes AKT1,RAC1,p53,epidermal growth factor receptor(EGFR),MTA1 and RARRES1 were significantly increased(all P<0.05).Conclusions MMP-8 protein can enhance the proliferation and migration ability of esophageal squamous cell carcinoma EC109 cells.MMP-8 overexpression can promote the mRNA expression of proliferation and migration related genes,which may be related to its promotion of EC109 cell proliferation and migration.

关 键 词：基质金属蛋白酶8 食管鳞癌 细胞增殖 细胞迁移 

分 类 号：R735.1[医药卫生—肿瘤]