Impaired autophagy activity-induced abnormal differentiation of bone marrow stem cells is related to adolescent idiopathic scoliosis osteopenia  

作　　者：Hongqi Zhang Guanteng Yang Jiong Li Lige Xiao Chaofeng Guo Yuxiang Wang 

机构地区：[1]Department of Spine Surgery and Orthopaedics,Xiangya Hospital,Central-South University,Changsha,Hunan 410008,China [2]National Clinical Research Center for Geriatric Disorder,Xiangya Hospital,Central-South University,Changsha,Hunan 410008,China

出　　处：《Chinese Medical Journal》2023年第17期2077-2085,共9页中华医学杂志（英文版）

基　　金：National Natural Science Foundation of China(No.82072390);Natural Science Foundation of Hunan,China(No.2020JJ4873)

摘　　要：Background:Osteopenia has been well documented in adolescent idiopathic scoliosis(AIS).Bone marrow stem cells(BMSCs)are a crucial regulator of bone homeostasis.Our previous study revealed a decreased osteogenic ability of BMSCs in AIS-related osteopenia,but the underlying mechanism of this phenomenon remains unclear.Methods:A total of 22 AIS patients and 18 age-matched controls were recruited for this study.Anthropometry and bone mass were measured in all participants.Bone marrow blood was collected for BMSC isolation and culture.Osteogenic and adipogenic induction were performed to observe the differences in the differentiation of BMSCs between the AIS-related osteopenia group and the control group.Furthermore,a total RNA was extracted from isolated BMSCs to perform RNA sequencing and subsequent analysis.Results:A lower osteogenic capacity and increased adipogenic capacity of BMSCs in AIS-related osteopenia were revealed.Differences in mRNA expression levels between the AIS-related osteopenia group and the control group were identified,including differences in the expression of LRRC17,DCLK1,PCDH7,TSPAN5,NHSL2,and CPT1B.Kyoto Encyclopedia of Genes and Genomes enrichment analyses revealed several biological processes involved in the regulation of autophagy and mitophagy.The Western blotting results of autophagy markers in BMSCs suggested impaired autophagic activity in BMSCs in the AIS-related osteopenia group.Conclusion:Our study revealed that BMSCs from AIS-related osteopenia patients have lower autophagic activity,which may be related to the lower osteogenic capacity and higher adipogenic capacity of BMSCs and consequently lead to the lower bone mass in AIS patients.

关 键 词：Adolescent idiopathic scoliosis Bone marrow stem cell AUTOPHAGY OSTEOPENIA 

分 类 号：R682.3[医药卫生—骨科学]