沉默驱动蛋白KIF4A在甲状腺癌中的表达及临床意义  

Expression and clinical significance of silencing kinesin KIF4A in thyroid cancer

作　　者：韩悦[1] 陈靖 王宝喜 李娜[4] Han Yue;Chen Jing;Wang Baoxi;Li Na(Department of Clinical Laboratory,the Second Hospital of Tianjin Medical University,Tianjin 300211,China;Department of Pancreatic Cancer,Tianjin Medical University Cancer Institute and Hospital,Tianjin 300060,China;Department of Clinical Laboratory,Hebei Yanda Hospital,Langfang 065201,China;Institute of Urology,the Second Hospital of Tianjin Medical University,Tianjin 300211,China)

机构地区：[1]天津医科大学第二医院检验科,天津300211 [2]天津医科大学肿瘤医院胰腺肿瘤科,天津300060 [3]河北燕达医院检验科,廊坊065201 [4]天津市泌尿外科研究所,天津300211

出　　处：《国际生物医学工程杂志》2022年第5期403-408,429,共7页International Journal of Biomedical Engineering

摘　　要：目的研究沉默驱动蛋白KIF4A在甲状腺癌组织中的表达情况以及与甲状腺癌患者临床病理特征的关系,并评估KIF4A在甲状腺癌进展过程中的作用。方法通过基因表达交互分析(GEPIA)数据库在线分析KIF4A在正常甲状腺组织和甲状腺癌人群中的表达情况以及与患者无病生存期的关系,通过免疫组化实验评估甲状腺癌患者的肿瘤组织及癌旁组织中KIF4A的表达。根据染色强度,将患者分为高表达组和低表达组,分析KIF4A的表达情况与临床病理特征的相关性。通过克隆形成实验和MTT实验探究KIF4A对甲状腺癌细胞增殖的影响。结果根据网络数据库分析,KIF4A在人甲状腺癌组织中呈明显高表达,高表达患者的无病生存期明显偏低。收集病例分析表明KIF4A表达强度与甲状腺癌患者的性别、年龄、淋巴结转移均无相关性(均P>0.05),与TNM分期和腺内播散具有相关性(均P<0.05)。克隆形成实验和MTT实验结果表明,KIF4A的表达促进了甲状腺癌细胞增殖(P<0.05)。结论KIF4A可以促进甲状腺癌的进展,有可能成为甲状腺癌新的治疗靶点。Objective To investigate the expression of silencing kinesin KIF4A in thyroid cancer tissues and its relationship with clinicopathological characteristics of thyroid cancer patients,and to assess the role of KIF4A in the progression of thyroid cancer.Methods The expression of KIF4A in normal thyroid tissues and the thyroid cancer population and its relationship with disease-free survival of patients were analyzed online by gene expression interaction analysis(GEPIA)database,and the expression of KIF4A in tumor tissues and paraneoplastic tissues of thyroid cancer patients was assessed by immunohistochemical assays.The patients were divided into high-and low-expression groups according to the staining intensity,and the correlation between the expression of KIF4A and clinicopathological features was analyzed.The effect of KIF4A on the proliferation of thyroid cancer cells was explored by a clone formation assay and an MTT assay.Results According to the analysis of the web-based database,KIF4A showed significantly high expression in human thyroid cancer tissues,and disease-free survival was significantly lower in highly expressed patients.The results of the case analysis showed that the correlation between KIF4A expression intensity and gender,age,and lymph node metastasis in thyroid cancer patients was not statistically significant(all P>0.05),and the correlation with TNM stage and intraglandular dissemination was statistically significant(all P<0.05).The results of the colony formation assay and the MTT assay showed that the expression of KIF4A promoted the proliferation of thyroid cancer cells(P<0.05).Conclusions KIF4A can promote the progression of thyroid cancer and has the potential to become a new therapeutic target for thyroid cancer.

关 键 词：沉默驱动蛋白KIF4A 甲状腺癌 靶点 

分 类 号：R736.1[医药卫生—肿瘤]