CD155在泡球蚴感染小鼠肝细胞凋亡和肝纤维化中的作用研究  

Role of CD155 in hepatocyte apoptosis and liver fibrosis induced by infection in mice

作　　者：史俊杰 努尔拜提·库苏曼 杨宁 毕晓娟 林仁勇 张爱莲[1] Shi Junjie;Nuerbaiti Kusuman;Yang Ning;Bi Xiaojuan;Lin Renyong;Zhang Ailian(Xinjiang Key Laboratory of Biological Resources and Genetic Engineering College of Life Science and Technology,Xinjiang University,Urumqi 830046,China;State Key Laboratory of Pathogenesis,Prevention,and Treatment of Diseases Highly Prevalent in Central Asia,Clinical Medical Research Institute,the First Hospital Affiliated with Xinjiang Medical University,Urumqi 830054,China)

机构地区：[1]新疆大学生命科学与技术学院,新疆生物资源与基因工程重点实验室,乌鲁木齐830046 [2]新疆医科大学第一附属医院临床医学研究院,省部共建中亚高发病成因与防治国家重点实验室,乌鲁木齐830054

出　　处：《国际生物医学工程杂志》2022年第5期384-389,394,共7页International Journal of Biomedical Engineering

基　　金：国家自然科学基金(82060371);新疆维吾尔自治区"天山雪松"计划(2019XS13)

摘　　要：目的探讨CD155在泡球蚴感染小鼠肝细胞凋亡和肝纤维化中的作用。方法将36只雌性C57BL/6小鼠随机分为假手术组和模型组,每组各18只。模型组小鼠经门静脉注射原头蚴的方法建立泡球蚴感染动物模型,假手术组小鼠注射等量生理盐水。分别于造模后1、3、6个月处死小鼠,收集肝脏。采用苏木素-伊红染色评价小鼠肝脏组织病理学变化,天狼星红染色检测肝脏纤维化情况,免疫组织化学方法检测肝细胞中CD155和含半胱氨酸的天冬氨酸蛋白水解酶-3(Caspase-3)的表达情况,并采用Person分析肝细胞中CD155的表达与Caspase-3和肝纤维化水平的相关性。结果模型组小鼠肝脏中可见明显病灶并伴随严重肝纤维化;与假手术组相比,模型组不同时期的小鼠肝纤维化水平显著升高,差异均有统计学意义(P<0.05);与假手术组相比,模型组不同时期的小鼠肝细胞中Caspase-3和CD155的表达均显著升高,差异有统计学意义(均P<0.05)。相关性分析显示肝细胞中CD155的表达与Caspase-3的表达呈正相关(r=0.9568;P<0.001;95%CI:0.8855~0.9841);肝细胞中CD155的表达和肝纤维化水平呈正相关(r=0.8539;P<0.001;95%CI:0.6437~0.9443)。结论泡球蚴感染小鼠不同时期肝细胞中CD155表达显著上调,并与肝细胞凋亡和肝纤维化水平呈正相关,提示CD155可能参与了泡球蚴感染所致肝细胞凋亡和肝纤维化过程。Objective To investigate the role of CD155 in hepatocyte apoptosis and liver fibrosis in mice infected with Echinococcus multilocularis.Methods Thirty-six female C57BL/6 mice were randomly divided into a sham surgery group and a model group,with 18 mice in each group.Mice in the model group were injected with protoscolex via the portal vein to create an animal model of E.multilocularis infection.Mice in the sham surgery group were injected with the same amount of saline.The mice were sacrificed at 1 month,3 months,and 6 months after modeling,and liver samples were collected.Hepatic pathological changes were observed by hematoxylin-eosin staining.Liver fibrosis was detected by Sirius red staining,and expression of Caspase-3 and CD155 in hepatocytes was detected by immunohistochemical staining.The correlation between CD155 expression in hepatocytes and Caspase-3 and liver fibrosis levels were analyzed by Person.Results There were obvious lesions in the liver of the model group accompanied by severe liver fibrosis.Compared with the sham surgery group,the expression of CD155 and Caspase-3 in mouse hepatocytes at different stages in the model group was significantly increased,and the differences were statistically significant(P<0.05).The model group's liver fibrosis level was significantly higher at different stages than the sham surgery group,with statistical significance(P<0.05).In addition,correlation analysis showed that expression of CD155 in hepatocytes was positively correlated with the expression of Caspase-3(r=0.9568;P<0.001;95%CI:0.8855-0.9841)and that expression of CD155 in hepatocytes was positively correlated with the degree of liver fibrosis(r=0.8539;P<0.001;95%CI:0.6437-0.9443).Conclusions CD155 expression was significantly up-regulated in mouse hepatocytes infected with E.multilocularis at different stages,which was positively correlated with the degree of hepatocyte apoptosis and liver fibrosis,suggesting that CD155 may be involved in the process of hepatocyte apoptosis and liver fibrosis caused by E.

关 键 词：CD155 泡球蚴 细胞凋亡 肝纤维化 含半胱氨酸的天冬氨酸蛋白水解酶-3 

分 类 号：R575[医药卫生—消化系统]