Actions of N-acetylcysteine,daptomycin,vancomycin,and linezolid on methicillin-resistant Staphylococcus aureus biofilms in the ventriculoperitoneal shunt infections:an experimental study  

作　　者：Tuba Kuruoglu Gamze Altun Enis Kuruoglu Derya BayırlıTuran Mehmet EminÖnger 

机构地区：[1]Department of Infectious Diseases and Clinical Microbiology,Medical Faculty,Ondokuz Mayıs University,Samsun,Turkey [2]Department of Histology and Embryology,Medical Faculty,Ondokuz Mayıs University,55139 Samsun,Turkey [3]Department of Neurosurgery,Medical Faculty,Ondokuz Mayıs University,Samsun,Turkey [4]Department of Infectious Diseases and Clinical Microbiology,School of Medicine,Yeni Yuzyil University,Istanbul,Turkey [5]Department of Neuroscience,Health Science Institute,Ondokuz Mayıs University,Samsun,Turkey

出　　处：《Chinese Neurosurgical Journal》2022年第4期239-247,共9页中华神经外科杂志（英文）

摘　　要：Background:Shunt systems are used to provide cerebrospinal fluid drainage in the treatment of hydrocephalus.Recently,antibiotic-impregnated shunt systems are used to prevent colonization in the ventriculoperitoneal catheters.Methicillin-resistant Staphylococcus aureus(MRSA)is the most common causative microorganism of shunt infections.The aim of the study is to investigate effects of several substances on MRSA biofilms in the ventriculoperitoneal catheters.Methods:The present study consists of mainly eight groups(each has two subgroups as antibiotic-impregnated and nonantibiotic-impregnated catheters).In addition,each group contains six molds using MRSA strains.In this study,daptomycin(DAPT)(2 mg/ml),vancomycin(VAN)(10 mg/ml),linezolid(LIN)(2 mg/ml),N-acetylcysteine(NAC)(6 mg/ml),and various combinations of these substances were used to evaluate the treatment against MRSA using scanning electron microscope(SEM)images and microbiological enumeration.Results:The colony count in the antibiotic-impregnated samples significantly decreased compared to nonantibiotic-impregnated samples in the MRSA,MRSA+DAPT,and MRSA+LIN groups(p<0.01),respectively.Conversely,the colony count in antibiotic-impregnated samples significantly increased compared to nonantibiotic-impregnated samples in NAC+DAPT and NAC+VAN groups(p<0.01),respectively.Conclusions:The results showed that the use of antibiotic-impregnated catheters has a significant impact on the prevention of infection whereas the combination of NAC and DAPT showed better antibiofilm and antibacterial effects than other combinations on the prevention and treatment of nonantibiotic-impregnated catheter infections.

关 键 词：METHICILLIN-RESISTANT Biofilm Antibiotic-impregnated catheters Scanning electron microscope 

分 类 号：R742.7[医药卫生—神经病学与精神病学] R446.5[医药卫生—临床医学]