Preparation of Xylo-oligosaccharide Based on Enzyme Adsorption-catalytic Coupling Technology  

基于酶吸附-催化耦合技术的低聚木糖制备研究

作　　者：CUI Enzhong ZHANG Qingming 

机构地区：[1]DepartmentofPharmacy,EasternTheaterCommandGeneralHospital,Nanjing210002,China

出　　处：《外文科技期刊数据库(文摘版)医药卫生》2021年第12期1038-1042,共8页

摘　　要：Objective: to improve the production efficiency and recovery of xylo-oligosaccharide by optimizing the adsorption and hydrolysis conditions of corn cob solid residue by fusion enzyme. Xylose oligosaccharide was prepared by catalytic coupling adsorption enzyme. Results the optimal adsorption conditions were substrate concentration 5%, pH 7.0, temperature 20℃ and adsorption time 30min. Under the optimal conditions, the adsorption rate of dissolved protein was 90.3%. The optimum enzymolysis conditions were ph7.0, temperature 50℃ and reaction time 24h. The yield of xylitol was 47.7% and the average degree of polymerization was 2.3. TLC analysis showed that xylitol, iron xylose, tetraxylose and amyl ester sugar were contained in the enzymolysis products. The quantitative results of HPLC showed that the contents of xylitol and xylanase were 45.2% and 36.7%, respectively. Conclusion: this study will not only provide a basis for the new technology of adsorption-catalytic coupling to produce xylo-oligosaccharides, but also provide a reference for other preparation methods of oligosaccharides.

关 键 词：XYLO-OLIGOSACCHARIDE enzyme adsorption-catalytic coupling fusion protein conditions optimization 

分 类 号：O658[理学—分析化学]