人外周血淋巴细胞体外刺激活化的原子力显微镜研究  

Observation of the activation of human peripheral blood lymphocytes with Atomic Force Microscopy

作　　者：廖问陶[1] 钟丽云[1] 查庆兵[1] 王云起[1] 李国有[1] 王小平[1] 何贤辉[1] 蔡继业[1] 

机构地区：[1]暨南大学化学系,广州510632

出　　处：《激光杂志》2006年第5期89-90,共2页Laser Journal

基　　金：国家自然科学重点项目(30230350);广东省自然科学基金(36704);国家自然科学基金(60578025);国家自然科学基金对外交流与合作项目(30540420311)赞助。

摘　　要：本文利用原子力显微镜,对分离的人外周血淋巴细胞和经多克隆刺激剂佛波醇酯(PDB)和离子霉素(Ion)刺激活化的淋巴细胞形态和膜表面超微结构的形态学变化进行了研究。结果表明:淋巴细胞在受刺激后形貌发生明显变化,尺寸变大,同时细胞表面超微结构也趋复杂,出现免疫突触。这些形态变化是与淋巴细胞的功能相适应的,AFM是研究淋巴细胞形态学变化和细胞结构功能之间关系的有力工具。To investigate the morphology and ultrastructure of human peripheral blood lymphocytes before and after activation with atomic force microscopy(AFM).Methods:Human resting lymphocytes were separated and activated by PDB and Ion,the cells before activation,activated for 24h and 48h were all scanned by AFM in air.Result: Lymphocytes were obvious enlarged and have a more complicated surface after activation,small granules and immunological synapse were found on the activated lymphocytes.Conclusion: AFM is a pow...

关 键 词：AFM 淋巴细胞 超微结构 

分 类 号：R392.1[医药卫生—免疫学]