^(188)Re标记抗人前列腺癌单克隆抗体及其在荷瘤裸鼠体内的放射性分布  

^(188)Re labeling of prostate cancer monoclonal antibody and its biodistribution in tumor-bearing nude mice

作　　者：张勇[1] 陈维真[1] 卢汉平[2] 梁昌盛[2] 刘长征[2] 

机构地区：[1]中山大学附属第三医院核医学科,广州510630 [2]中山大学基础医学院实验核医学教研室,广州510080

出　　处：《核技术》2007年第8期699-701,共3页Nuclear Techniques

基　　金：广东省科技计划项目(2003B30103)

摘　　要：为探讨188Re标记抗前列腺癌单克隆抗体7E11C5.3的方法及其生物学分布,采用2-巯基乙醇直接还原法制备188Re-7E11C5.3标记物,常规测定标记率和免疫活性,在荷瘤裸鼠体内检测标记物的生物学分布。结果显示,188Re-7E11C5.3的标记率为(93.16±2.18)%,免疫活性分数为(74.86±1.86)%;经尾静脉注入裸鼠体内后,肿瘤组织摄取率在24h达高峰,非肿瘤组织在4h摄取率较高,以后均随时间延长逐步降低;血液放射性清除迅速;24h时T/NT值达最大,至72h仍可保持在较高水平。结果表明,188Re-7E11C5.3在裸鼠体内对前列腺癌移植瘤有良好的靶向定位作用,可用于前列腺癌放射免疫显像和治疗研究。To investigate radiolabeled monoclonal antibody 7E11C5.3 against human prostate cancer with 188Re and its biodistribution, 188Re-7E11C5.3 was prepared by direct 2-mercaptoethanol-reduction method. Labeling efficiency was measured by paper chromatography. Biodistribution of 188Re-7E11C5.3 was studied in nude mice xenografted with human prostate cancer. The labeling yield of 188Re-7E11C5.3 was 93.16±2.18%. The immunoreactive fraction of 188Re-7E11C5.3 was 74.86±1.86%. After 188Re-7E11C5.3 was injected through...

关 键 词：前列腺肿瘤 抗体 单克隆抗体 ^(188)Re 

分 类 号：R737.25[医药卫生—肿瘤]