中间偃麦草NPR1同源基因TiNH1的分离和特性分析  

Isolation and Characterization of NPR1 Homolog Gene TiNH1 in Thinopyrum intermedium

作　　者：唐益苗[1] 张增艳[1] 辛志勇[1] 

机构地区：[1]中国农业科学院作物科学研究所,农作物基因资源与基因改良国家重大科学工程,农业部作物遗传育种重点开放实验室,北京100081

出　　处：《中国农业科学》2007年第6期1101-1107,共7页Scientia Agricultura Sinica

基　　金：国家"863"计划(2003AA211010;2004AA222120)

摘　　要：【目的】NPR1是调控植物抗病反应的一个关键基因。对中间偃麦草NPR1同源基因TiNH1进行分离和特性分析。【方法】利用RT-PCR和RACE(rapid amplification of cDNA ends)技术获得TiNH1全长cDNA序列,利用Northern和Southern分别研究TiNH1表达特性及其在中间偃麦草基因组中存在形式。【结果】获得了该基因cDNA序列,命名为TiNH1。其编码蛋白TiNH1的氨基酸序列分别与水稻、烟草和拟南芥的NPR1同源性为80%、54%和46%。Northern杂交分析结果表明:TiNH1基因在正常情况下有微量表达,在小麦白粉病菌和纹枯病菌诱导下,该基因表达水平得到提高。Southern杂交分析结果表明;该基因以单拷贝形式存在于中间偃麦草基因组中。【结论】中间偃麦草NPR1同源基因TiNH1编码由580个氨基酸组成的蛋白质TiNH,具有已知NPR1蛋白保守的结构域和功能氨基酸,可能参与寄主对小麦白粉病菌和纹枯病菌的防御反应。【Objective】NPR1 is a key regulator in plant defense response. The NPR1 homolog gene TiNH1, named as TiNH1 was isolated from Th. intermedium and characterized. 【Method】The full-length cDNA of TiNH1 gene was isolated by RT-PCR and RACE (rapid amplification of cDNA ends ) methods. The expression pattern of TiNH1was studied by Northern blot and its copy numbers in Th. intermedium genome was clarified by Southern blot.【 Result】The full-length cDNA of TiNH1 was obtained from Th. intermedium. The predicted protein...

关 键 词：中间偃麦草 NPR1同源基因 白粉病菌 纹枯病菌 抗性 

分 类 号：S332.2[农业科学—作物遗传育种]