荷叶黄酮化合物对胰脂肪酶抑制作用的研究  

Inhibitory Effect on Pancreatic Lipase of Flavonoids Derived from Lotus Leaf

作　　者：霍世欣[1] 周陶忆[1] 司晓晶[1] 吕敬慈[1] 

机构地区：[1]上海大学理学院,上海200444

出　　处：《天然产物研究与开发》2008年第2期328-331,共4页Natural Product Research and Development

摘　　要：荷叶粗粉用60%乙醇回流提取,经石油醚脱脂,再用聚酰胺柱色谱分离得到不同部位的黄酮提取物。通过比较50%乙醇洗脱部位的黄酮提取物和市售减肥药奥利司他(Orlistat)对胰脂肪酶的抑制作用,得到它们的半抑制率IC50分别为0.0076,0.0048mg/mL。通过Lineweaver-Burk图和Dixon图的特征,表明该抑制剂对胰脂肪酶的抑制呈非竞争性抑制,抑制常数Ki=0.0147mg/mL,米氏常数Km=42.2mg/mL。The powder of lotus leaf was extracted with 60% ethanol completely,and then the mixtures were degreased using petroleum ether.Finally,the various fractions of flavonoids were separated and purified from the extractions by polyamide column chromatography.The effects of 50% ethanol eluted flavonoids extraction and orlistat on the enzyme activity of pancreatic lipase were studied.It was found that the half-inhibition concentrations (IC50) of them were 0.0076 and 0.0048 mg/mL,respectively.Based on the Dixon plo...

关 键 词：荷叶 黄酮 胰脂肪酶 抑制剂 

分 类 号：R285[医药卫生—中药学]