阳离子脂质体-非编码质粒DNA复合物抗小鼠结肠癌的实验研究  

Treatment of Murine Colon Cancer with Complexes of Cationic Liposome and Noncoding Plasmid DNA

作　　者：王中瑜[1] 汤明海[1] 陈平[1] 邓洪新[1] 付译节[1] 

机构地区：[1]四川大学华西医院生物治疗国家重点实验室,四川成都610041

出　　处：《华西医学》2007年第4期813-816,共4页West China Medical Journal

基　　金：受国家"973"项目(NO:2004CB518807)资助

摘　　要：目的:探讨利用阳离子脂质体-非编码质粒DNA复合物治疗小鼠结肠癌以及作用机制。方法:建立小鼠结肠癌肺转移和皮下肿瘤模型,静脉给予阳离子脂质体-非编码质粒DNA复合物进行治疗,测量瘤体大小,计数肺转移瘤的数量,观察各个脏器的病理学改变。结果:经阳离子脂质体-非编码质粒DNA复合物治疗后,治疗组的肺转移瘤的数量(肺转移模型中)和瘤体体积(皮下肿瘤模型中)明显低于对照组,治疗组小鼠的脾脏中巨核细胞数量明显增多。结论:阳离子脂质体-非编码质粒DNA形成的复合物具有治疗小鼠结肠癌的作用。Objective:To investigate the anti-tumor effect of complexes of cationic lipid and noncoding plasmid DNA in murine colon cancer.Methods:Murine lung metastasis model was established by intravenous injection of CT26(murine colon carcinoma cell line)cells into BALB/c mice,while subcutaneous tumor model was established by subcutaneously injection.The tumor-bearing mice were divided into three groups and injected intravenously with one of the following preparations:cationic lipid/nocoding plasmid DNA complex,cati...

关 键 词：阳离子脂质体 非编码质粒DNA 结肠癌 基因治疗 

分 类 号：R735.35[医药卫生—肿瘤]