二甲基亚砜拮抗烹调油烟冷凝物遗传毒性的研究  

Antagonism of DMSO against Genotoxicity of Cooking Oil Fume Condensate to BEAS-2B Cell

作　　者：黄波[1] 陈锋[1] 朱茂祥[2] 杨陟华[2] 张蓉芳 

机构地区：[1]南华大学公共卫生学院卫生学教研室,湖南衡阳421001 [2]军事医学科学院放射医学研究所,北京100850

出　　处：《环境与健康杂志》2007年第3期162-164,共3页Journal of Environment and Health

摘　　要：目的研究二甲基亚砜(DMSO)对烹调油烟冷凝物(COFC)致人支气管上皮细胞遗传毒性作用的影响。方法采用永生化人支气管上皮细胞系BEAS-2B细胞,分为空白对照组、DMSO对照组、试剂对照组(0.1%乙醇)、COFC染毒组(50、100、150mg/L)和相应的DMSO保护组(0.5%DMSO)。采用单细胞凝胶电泳和微核、多核实验研究COFC的遗传毒性及DMSO对烹调油烟损伤的防护作用。结果COFC染毒组可引起细胞DNA断裂,细胞拖尾率、彗星尾部DNA含量、尾长、尾部面积和尾距增加,微核多核率升高,DMSO对以上损伤有显著的拮抗作用。结论COFC对人支气管上皮细胞具有明显的遗传毒性作用,而DMSO对此有抑制作用。Objective To study the antagonism of DMSO against the toxicity of cooking oil fume condensation to BEAS-2B cell.Methods The comet assay,micronucleus test and multinucleated cells test were used to research the genotoxicity induced by cooking oil fume condensation(COFC)and the antagonism of DMSO.Results COFC induced DNA broken,the tail area,rate of comet occurrence,tail length,tail moment,olive tailmoment increased significantly,the frequencies of micronucleus and multinucleated cells were significantly incr...

关 键 词：空气污染 室内 烹调油烟冷凝物 遗传毒性 人支气管上皮细胞 二甲基亚砜 

分 类 号：R114[医药卫生—卫生毒理学]