柑橘采后蒂腐病生防酵母菌的筛选及鉴定  

Screen and Identification of Biocontrol Yeast against Postharvest Stem-end Rot of Citrus

作　　者：陈丽锋[1] 闵晓芳[1] 邓伯勋[1] 余慧[1] 

机构地区：[1]华中农业大学国家柑橘育种中心/园艺植物生物学教育部重点实验室,武汉430070

出　　处：《中国生物防治》2007年第S1期47-52,共6页Chinese Journal of Biological Control

基　　金：湖北省科技攻关(2006AA201C54);国家自然科学基金(30371008)

摘　　要：通过离体及混菌试验,筛选出了对柑橘黑色蒂腐病具有较强拮抗作用的9株酵母菌。其中X-1、MA-2在离体条件下效果最好,在混菌5d后仍不见病原菌的生长。通过果实抑菌试验得到活体条件下效果最好的菌株Pg2-5,处理后果实的发病率在20.00%,比对照76.67%降低了56.67个百分点。防治效果与酵母菌的浓度呈正相关,与培养时间呈负相关。经形态和生理生化指标鉴定Pg2-5为假丝酵母属欧诺比假丝酵母。Nine yeast antagonists against postharvest stem-end rot disease of Citrus were screened by in vitro experiment,among which X-1,MA-2 played the best role,and pathogen didn t grow in the mixing experiment after 5 days.The strain Pg2-5 had the best effect on the disease during the following in vivo experiment;the disease was reduced by 56.67% to the control.And the biocontrol activity was positively correlated to the concentration of yeast antagonist,negatively correlated to the time of incubation.The strain P...

关 键 词：柑橘 蒂腐病 拮抗菌 生物防治 采后病害 

分 类 号：S436.661.12[农业科学—农业昆虫与害虫防治]