clusterin、Bax及p53在非小细胞肺癌中的表达及相关性研究  

Relationship of clusterin expression with Bax and p53 expression in non-small cell lung cancer

作　　者：牛林琳[1] 臧家兰[1] 蔡莉[1] 李春红[1] 曹鸿艳[1] 

机构地区：[1]哈尔滨医科大学附属肿瘤医院内科,150040

出　　处：《中国肺癌杂志》2007年第4期284-287,共4页Chinese Journal of Lung Cancer

基　　金：黑龙江省科委重大攻关课题(NoGB05C401-07);黑龙江省教育厅骨干教师项目(No1151G026)资助~~

摘　　要：背景与目的已有的研究表明簇集蛋白(clusterin)是近年新发现的凋亡相关因子,在多种肿瘤中上调表达,在肿瘤的发生发展中起重要作用。clusterin的抗凋亡作用与其他抗凋亡因子有相关性。本文旨在探讨clusterin、Bax和p53在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达情况及其与肺癌发生发展的关系。方法采用SP免疫组化方法和Western blot法,检测NSCLC组织中clusterin、Bax和p53的表达情况,并结合临床和病理资料进行分析。结果免疫组化显示clusterin在79.25%(42/53)的NSCLC组织中阳性表达。NSCLC组织中clusterin表达阳性率明显高于正常肺组织(χ2=23.68,P<0.05),与肿瘤的分化程度(rs=0.464,P<0.01)、临床分期(rs=0.320,P<0.01)及有无淋巴结转移(rs=0.255,P<0.05)呈正相关,但与年龄、性别及病理分型无关。clusterin与p53的表达呈正相关(rs=0.589,P<0.01),与Bax的表达呈负相关(rs=-0.346,P<0.01)。Western blot法显示clusterin在NSCLC组织中的表达水平明显高于正常肺组织(0.541±0.010比0.201±0.020,P<0.05)。结论clusterin可能通过抗凋亡机制在肺癌的生物学特性中发挥重要作用。Background and objective It has been proven that clusterin is a newly apoptosis-related factor and upregulates in many tumors. It plays important roles in carcinogenesis and tumor progression. The antiapoptosis of clusterin seems to be relative to other antiapoptosis factors. The aim of this study is to investigate the expression and significance of clusterin in non-small cell lung cancer (NSCLC) tissue. Methods The expression of clusterin, p53 and Bax in NSCLC were detected by immunohistochemical SP method and Western blot assay. Results Positive expression rate of clusterin was 79.25% (42/53) in NSCLC tissues which was much higher than that in normal lung tissue (2/16, 12.50%) (χ2=23.68, P<0.05). The expression of clusterin was closely related to pathological differentiation (rs=0.464, P<0.01), clinical stage (rs =0.320, P<0.01) and lymph node metastasis (rs=0.255, P<0.05), but not correlated to the sex (χ2=0.007, P>0.05), age (χ2=0.707, P>0.05) and histological type (χ2=0.702, P>0.05). The expression of clusterin in NSCLC was positively correlated to the expression of p53 (rs=0.589, P<0.01), but was negatively related to the expression of Bax (rs =-0.346, P<0.01). The relative expression level of clusterin protein in NSCLC was significantly higher than that in normal lung tissue (0.541±0.010 vs 0.201±0.020) (P<0.05). Conclusion Clusterin may play an important role in the biological characteristics of NSCLC by the antiapoptosis pathway.

关 键 词：非小细胞肺癌 簇集蛋白 P53 BAX 凋亡 免疫组化 

分 类 号：R734.2[医药卫生—肿瘤]