表皮钙黏蛋白mRNA表达水平与人NSCLC临床病理特征关系研究  

A study on the relationship between the level of E-cadherin mRNA expression and pathological characteristics of non-small cell lung cancer

作　　者：陈晓峰[1] 丁修敬[2] 杨倍[2] 包敏伟[2] 朱军[2] 王海峰[2] 徐志飞[2] 

机构地区：[1]第二军医大学附属长征医院胸外科,上海200433200003 [2]同济大学附属上海市肺科医院

出　　处：《中国肺癌杂志》2007年第6期471-476,共6页Chinese Journal of Lung Cancer

基　　金：上海市卫生局科技发展基金项目(No99424)资助~~

摘　　要：背景与目的钙黏蛋白在维持正常上皮细胞的连接起着重要的作用,表皮钙黏蛋白是钙黏蛋白家族中的一种,其表达降低可能与人类癌症的侵袭和去分化密切相关。本研究拟探讨非小细胞肺癌(NSCLC)中表皮钙黏蛋白mRNA表达水平与NSCLC病理分级和临床分期之间的关系。方法用RT-PCR方法检测53例NSCLC标本、46例癌旁肺组织、5例肺部良性结节性病变组织中表皮钙黏蛋白mRNA的表达水平,根据手术、病理和影像学结果判断有无转移并确定分期,分析表皮钙黏蛋白mRNA表达水平与临床变量之间的关系。结果表皮钙黏蛋白mRNA在NSCLC和癌旁肺组织中的阳性率分别为45.3%(24/53)和45.7%(21/46),P>0.05;低分化、晚期、肺门纵隔淋巴结转移的NSCLC,其表皮钙黏蛋白mRNA的表达量显著性降低(P<0.05);表皮钙黏蛋白mRNA表达阳性和阴性的患者中位生存期分别为15.5个月和46个月,其表达量与患者预后无显著相关性(P>0.05)。结论E-cadherin与NSCLC的淋巴结转移、分化及病理分期有关,但无证据表明其能影响NSCLC患者的预后。Background and objective E-cadherin is a subclass of the cadherin family that plays an important role in the maintenance of intercellular junctions in normal epithelium.Decreased expression of E-cadherin might be closely related to invasiveness and dedifferentiation in human cancers.There is increasing evidence that modulation of the E-cadherin-catenin cell-cell adhesion complex is an important step in the initiation and progression of human cancers.The aim of this study is to investigate the relationship between the level of E-cadherin mRNA expression and pathological grades and clinical stages of non-small cell lung cancer(NSCLC).Methods RT-PCR was used to measure the level of E-cadherin mRNA expression in 53 specimens of NSCLC,46 of para-cancer lung tissues,5 of benign nodal lung diseases,and the stages of disease was determined according to the results of surgery,pathology and imaging diagnoses.Then analyses were carried out between the level of E-cadherin mRNA expression and the clinical variables.Results 45.3%(24/53) and 45.7%(21/46) specimens of NSCLC and para-cancer lung tissue were positive for E-cadherin mRNA expression respectively(P>0.05);NSCLC with low differentiation,advanced stages and nodal metastases showed a magnificantly lower expression of E-cadherin mRNA(P<0.05).The median survival time for E-cadherin mRNA positive and negative patients were 15.5 months and 46 months,respectively,but the expression of E-cadherin mRNA did not correlate with patient's survival(P>0.05).Conclusion E-cadherin expression is related to the differentiation,lymph node metastasis and pathological staging of NSCLC,but probably does not effectively affect its prognosis.

关 键 词：肺肿瘤 表皮钙黏蛋白基因 MRNA表达 转移 

分 类 号：R734.2[医药卫生—肿瘤]