人抗原R与血管内皮生长因子-C在非小细胞肺癌中的表达及意义  

The expression of human antigen R and vascular endothelial growth factor-C and their significance in non-small cell lung cancer

作　　者：王俊[1] 郭燕[1] 章必成[2] 陈正堂[1] 高建飞[2] 赵勇[2] 向德兵[3] 

机构地区：[1]第三军医大学附属新桥医院全军肿瘤研究所,重庆400037 [2]广州军区武汉总医院肿瘤科 [3]第三军医大学附属大坪医院病理科,重庆400037

出　　处：《中国肺癌杂志》2007年第6期481-485,共5页Chinese Journal of Lung Cancer

基　　金：国家自然科学基金(No30371586)资助~~

摘　　要：背景与目的血管内皮生长因子-C(VEGF-C)是介导淋巴管生成的重要分子,参与了肿瘤淋巴道转移的过程。人抗原R(HuR)是最早发现的RNA结合蛋白之一,可增加多种生长因子、细胞因子mRNA的稳定性,从而上调蛋白质表达。本研究的目的是探讨HuR在非小细胞肺癌(NSCLC)中的表达及其与VEGF-C表达、肺癌临床病理学特征的关系。方法采用免疫组织化学方法(SP法)检测81例NSCLC组织和15例肺良性病变组织中HuR和VEGF-C的表达情况。结果免疫组织化学显示:81例NSCLC的HuR胞浆阳性表达率、HuR胞核阳性表达率、VEGF-C阳性表达率分别为45.7%(37/81)、82.7%(67/81)和70.4%(57/81),与肺良性病变组织比较均有显著性差异(P<0.05)。胞浆HuR表达水平与淋巴结转移、分化程度和pTNM分期密切相关(P<0.05),但与患者的性别、年龄、肺癌组织学类型无明显关系(P>0.05)。胞浆HuR高表达(P<0.05)而不是胞核HuR高表达(P>0.05)与VEGF-C的表达呈正相关。结论胞浆HuR和VEGF-C在肺癌组织中的表达与肿瘤进展有关。HuR可能参与了肿瘤细胞VEGF-C的表达调控。Background and objective Vascular endothelial growth factor-C(VEGF-C) plays a critical role in tumor-induced lymphangiogenesis and contributes to lymph node metastasis.Human antigen R(HuR) is one of the firstly identified RNA-binding proteins.It can increase the stability of a variety of growth factors and cytokines and upregulate protein expression.The aim of this study is to investigate the expression of HuR and VEGF-C protein in non-small cell lung cancer(NSCLC),and explore the relationship between the expression of HuR and VEGF-C and clinicopathological factors.Methods HuR and VEGF-C protein levels were detected in 81 NSCLC tissues and 15 control benign pulmonary lesion tissues by immunohistochemistry method(SP method).Results In NSCLC tissues,positive rate of cytoplasmic HuR,nuclear HuR and VEGF-C was 45.7%(37/81),82.7%(67/81) and 70.4%(57/81),respectively.There was a significant difference in positive expression of HuR and VEGF-C between NSCLC and benign pulmonary lesion tissues(P<0.05).The expression of cytoplasmic HuR was closely related to pTNM stages,differentiation degree and lymph node metastasis(P<0.05),but not correlated with sex,age and histological classification(P>0.05).Furthermore,cytoplasmic immunoreactivity for HuR protein(P<0.05) but not nuclear HuR expression(P>0.05) was associated with high VEGF-C expression.Conclusion Cytoplasmic HuR and VEGF-C are overexpressed in NSCLC,and are related to tumor development.HuR may mediate the modulation of VEGF-C gene expression in NSCLC.

关 键 词：人抗原R 血管内皮生长因子-C 非小细胞肺癌 免疫组织化学 

分 类 号：R734.2[医药卫生—肿瘤]