碱性成纤维细胞生长因子在酒精性肝病大鼠中的表达  

Expression of Basic Fibroblast Growth Factor in Rats with Alcoholic Liver Disease

作　　者：崔巍[1] 王颖[1] 林红[1] 傅宝玉[1] 

机构地区：[1]中国医科大学附属第一医院消化内科,辽宁省沈阳市110001

出　　处：《中国全科医学》2007年第12期985-986,996,共3页Chinese General Practice

摘　　要：目的探讨碱性成纤维细胞生长因子(bFGF)在酒精性肝病大鼠肝脏中的动态变化,以进一步研究酒精性肝病的发病机制。方法将45只大鼠随机分成两组,实验组大鼠采用乙醇灌胃法制备酒精性肝病大鼠模型。实验进行4、8、12周末分批处死动物,采用苏木素-伊红(HE)染色及Gieson氏染色观察肝脏组织学改变,并应用免疫组织化学技术及Western blot检测bFGF在酒精性肝病过程中的定位及表达。结果对照组大鼠bFGF主要存在于血管内皮细胞胞浆中,呈弱表达。至实验进行12周,实验组大鼠bFGF表达明显增强,存在于血管内皮细胞外、窦周细胞和纤维间隔内浸润细胞的胞浆及汇管区、纤维间隔区的细胞外基质中。Weston blot结果显示,实验组大鼠12周末bF-GF蛋白表达量与对照组比较,差别有统计学意义(P<0.05)。结论随着酒精性肝病的病程进展,bFGF表达量逐渐增强,它是一种促纤维化因子。Objective To investigated the expression of basic fibroblast growth factor (bFGF) in alcoholic liver disease in rats.Methods Wistar rats weighing between 160 g and 200 g were intragastricly fed with ethanol (8 to 10 g·kg-1·d-1) for 12 weeks.Control group received normal sodium (6.0 ml·kg-1·d-1).at the days of 4,8,12 weeks,rats were killed and received liver histopathological test.Expressions of bFGF in liver were examined.Results After 4 weeks,slight steatosis was observed in alcoholic rats.Up to 12 weeks,marked hepatic fatty changed,inflammation,necrosis and mild fibrosis were also observed.in control group,bFGF was weakly expressed in endothelial cells lining and vessels ,in study group,it was strongly expressed in sinusoidal cells and extracellular matrix of fibrous bands.The difference of bFGF expression between two groups at the day of 12 week was significant(P<0.05).Conclusion bFGF were highly expressioned in alcoholic rats.

关 键 词：碱性成纤维细胞生长因子 肝硬化 酒精性 大鼠 

分 类 号：R575.2[医药卫生—消化系统]