Wistar大鼠脑内各区的Salsolinol氮甲基转移酶活性检测  

Assessment of Salsolinol N-methyltransferase Activity in the Wistar Rat Brains

作　　者：牟晓玲[1] 张天[1] 彭博[1] 朱勇[1] 魏玉霞[1] 肖盛元[1] 邓玉林[1] 

机构地区：[1]北京理工大学生命科学与技术学院

出　　处：《分析测试学报》2007年第z1期87-88,92,共3页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(30170336)

摘　　要：Parkinson’s disease is a common chronic neurodegenerative disease.N-methylation can enhance the neurotoxiicity to dopaminergic neuron in the metabolic process of catechol isoquinolines and Salsolinol N-methyltransferases (SNMT) may play an important role in the pathogenesis of PD.A new method including SNMT purification and detecting SNMT activity was developed and displays an excellent sensitivity and stability.In addition,the SNMT activity in Wistar rats substantia nigra,striatum and cerebellum.Parkinson's disease is a common chronic neurodegenerative disease.N-methylation can enhance the neurotoxiicity to dopaminergic neuron in the metabolic process of catechol isoquinolines and Salsolinol N-methyltransferases (SNMT) may play an important role in the pathogenesis of PD.A new method including SNMT purification and detecting SNMT activity was developed and displays an excellent sensitivity and stability.In addition,the SNMT activity in Wistar rats substantia nigra,striatum and cerebellum.

关 键 词：Salsolinol N-methyltransferases HPLC-ESI/TOF 

分 类 号：O657-55[理学—分析化学]