液相色谱-串联质谱法测定牛尿中多种非类固醇同化激素残留量  

Determination of Residue Nonsteroid Anabolic Hormones in Bovine Urine by Liquid Chromatography-Tandem Mass Spectrometry

作　　者：许泓[1] 庞国芳[2] 林安清[1] 古珑[1] 何佳[1] 张曼[1] 张骏[1] 林娜[1] 

机构地区：[1]天津出入境检验检疫局,天津300201 [2]秦皇岛出入境检验检疫局,河北秦皇岛066002

出　　处：《分析测试学报》2007年第z1期93-95,共3页Journal of Instrumental Analysis

摘　　要：Bovine urine was centrifuged.Added the internal standard(ZER-d4 and DES-d8) into the supernate.The extract was cleaned up by two immunoaffinity columns respectively.A C8 analytical column was used and the analytes were chromatographed by acetonitrile-water(70:30,v/v).With HPLC-MS/MS,data acquisition was achieved by using atmospheric pressure chemical ionization (APCI) in negative scan mode and multiple reaction monitoring (MRM) determination mode.Quantification was made by internal standarad method.The limits of determination of DES and DEN are 0.05 ng·g-1,the recovery level was 80 %-106% between 0.5 and 10 ng·g-1.The limits of determination of HEX and ZER are 0.025 ng·g-1,the recovery level was 83%-104% between 0.25 and 5 ng·g-1.Bovine urine was centrifuged.Added the internal standard(ZER-d4 and DES-d8) into the supernate.The extract was cleaned up by two immunoaffinity columns respectively.A C8 analytical column was used and the analytes were chromatographed by acetonitrile-water(70:30,v/v).With HPLC-MS/MS,data acquisition was achieved by using atmospheric pressure chemical ionization (APCI) in negative scan mode and multiple reaction monitoring (MRM) determination mode.Quantification was made by internal standarad method.The limits of determination of DES and DEN are 0.05 ng·g-1,the recovery level was 80 %-106% between 0.5 and 10 ng·g-1.The limits of determination of HEX and ZER are 0.025 ng·g-1,the recovery level was 83%-104% between 0.25 and 5 ng·g-1.

关 键 词：HPLC-MS/MS Bovine urine Nonsteroid anabolic hormones RESIDUE 

分 类 号：O657-55[理学—分析化学]