高效液相色谱-串联质谱法测定食品添加剂中蛇床子素含量  

Determination of Osthole in Food Additive by High-Performance Liquid Chromatography-Tandem Mass Spectrometry

作　　者：李昱[1] 吴胜明[1] 方均建[1] 马昆鹏[1] 宋占军[1] 董方霆[1] 

机构地区：[1]国家生物医学分析中心,北京100850

出　　处：《分析测试学报》2007年第z1期242-243,247,共3页Journal of Instrumental Analysis

摘　　要：To establish HPLC electrospray ionization mass spectrometry (HPLC-ESI MS) method for determination of osthole in food additive.The samples were dipped in methanol overnight and extracted with it by ultrasonic instrument,then the supernatant was send to sampler for detection.Sample was chromatographed using 0.4%HAc solution-MeOH (35:65,by volume) mobile phase on an Xterra MS C18 column.Analyte determination was performed by ESI MS/MS in the multiple reaction monitoring(MRM)mode.The established method of LC-MS/MS determining the concentration of osthole in food additive has the characteristic of strong specificity,high sensitivity and good reproducibility.The linear range of papaverine was 0.001-10 μg/mL,method recovery was 97.42%,RSD was 8.37%,the limit of detection was 1 ng/mL and the limit of quantitation was 0.001 μg/mL.To establish HPLC electrospray ionization mass spectrometry (HPLC-ESI MS) method for determination of osthole in food additive.The samples were dipped in methanol overnight and extracted with it by ultrasonic instrument,then the supernatant was send to sampler for detection.Sample was chromatographed using 0.4%HAc solution-MeOH (35:65,by volume) mobile phase on an Xterra MS C18 column.Analyte determination was performed by ESI MS/MS in the multiple reaction monitoring(MRM)mode.The established method of LC-MS/MS determining the concentration of osthole in food additive has the characteristic of strong specificity,high sensitivity and good reproducibility.The linear range of papaverine was 0.001-10 μg/mL,method recovery was 97.42%,RSD was 8.37%,the limit of detection was 1 ng/mL and the limit of quantitation was 0.001 μg/mL.

关 键 词：OSTHOLE Liquid chromatography-tandem mass spectrometry(LC-MS/MS) 

分 类 号：O657-55[理学—分析化学]