MMP-2在膀胱移行细胞癌组织中表达水平与肿瘤血管形成的关系  

Study of MMP-2 Expression and its Roles in Angiogenesis of Bladder Transitional Cell Carcinoma

作　　者：卢洪凯[1] 周全[1] 李刚[1] 薄学军[1] 刘志明[1] 刘鲁东[1] 

机构地区：[1]潍坊医学院附属潍坊市人民医院泌尿外科,山东潍坊261041

出　　处：《潍坊医学院学报》2007年第1期31-34,Ⅲ,共5页Acta Academiae Medicinae Weifang

基　　金：潍坊市科技发展计划资助课题(课题编号:2005第3号课题)

摘　　要：目的探讨MMP-2在膀胱移行细胞癌血管形成中的作用,为抑制膀胱肿瘤的血管生成疗法提供理论依据。方法采用免疫组化法(S-P)检测48例膀胱移行细胞癌及9例正常膀胱组织中MMP-2的表达,计算机图像光密度分析,根据阳性信号面积/检测总面积的方法,以10%为阳性判断标准,计算阳性率。采用血管内皮细胞单克隆抗体CD34进行血管内皮染色,低倍镜下寻找肿瘤组织中微血管密度最密集的部位(“热区”),然后在高倍镜下计数热区内的平均微血管密度(MVD),结合临床资料及统计学处理分析以评价MMP-2在膀胱移行细胞癌血管生成中的作用。结果48例膀胱移行细胞癌组织中,MMP-2在膀胱移行细胞癌组织中的阳性表达率为64.6%(31/48),而9例正常膀胱组织表达阴性,两者差别有显著性(P<0.01),MMP-2表达水平与患者的年龄、性别、肿瘤数目、直径、病理分级等无关(P>0.05),而在病理分期之间(Tis^T1与T2~T4)差别有显著性(P<0.05),浸润性膀胱移行细胞癌明显高于浅表性膀胱移行细胞癌;MMP-2的表达与MVD成正相关(r=0.592,P<0.05),随着MMP-2表达增加,MVD也增加。结论MMP-2在膀胱移行细胞癌组织中高表达,MMP-2与MVD呈正相关,促进了膀胱移行细胞癌新生血管的形成。Objective To study MMP-2 role in the angiogenesis of bladder transitional cell carcinoma(BTCC),meanwhile providing theoretical bases for the new therapy of inhibiting angiogenesis of BTCC.Methods Using immunohistochemistry(S-P),we examined the expression of MMP-2 in 48 cases of BTCC and 9 cases of normal bladder tissues by computer-assisted image analysis,and by monoclonal antibody of CD34 to enumerate the number of vascular.At first,we found the most dense area of BTCC in the low-amplified microscope('hot area') and then enumerated the mean microvascular density(MVD) in the high-amplified microscope,and evaluated the MMP-2 role in the angiogenesis of bladder transitional cell carcinoma(BTCC) and relationship by analyzing clinical data and statistics process.Results In all the 48 cases of BTCC,the positive rate of MMP-2 was 64.6%(31/48),but there was no expression in 9 cases of normal bladder tissue.The expression of MMP-2 was not related to patients' age,sex,size,number,grade(P>0.05),but to the clinical stage(Tis～T1 and T2～T4)(P<0.05),and its expression in invasive BTCC was more significant than that in superficial BTCC.Meanwhile,the expression of MMP-2 was positively related to MVD(r=0.592,P<0.05).Conclusion There are high expression of MMP-2 in the BTCC tissues,which is positively related to MVD,may promote the angiogenesis of BTCC.

关 键 词：膀胱移行细胞癌 基质金属蛋白酶 新生血管化 微血管密度 

分 类 号：R737.14[医药卫生—肿瘤]