SBHL对人宫颈癌细胞ME180增殖和凋亡作用的实验研究  

The experimental investigation derived from Sdosodine Hydrochlorida on apoptosis of the cervical cancer ME180 cells

作　　者：于秀艳[1] 艾金霞[2] 

机构地区：[1]吉林省肿瘤医院,长春130012 [2]北华大学医学院免疫教研室,吉林132013

出　　处：《中国免疫学杂志》2007年第12期1087-1089,共3页Chinese Journal of Immunology

摘　　要：目的:研究SBHL对人宫颈癌细胞的增殖抑制作用和作用机理。方法:用MTT法检测SBHL对ME180细胞增殖,用流式细胞技术和琼脂凝胶电泳法分析细胞凋亡、细胞周期变化,用电子显微镜观察细胞超微结构的变化。结果:SBHL作用ME180细胞24小时后,细胞存活率明显下降,而且随着澳洲茄胺盐酸盐浓度的增加,抑制作用增强(P<0.01);SBHL可将ME180细胞周期阻滞于G1期,诱导细胞凋亡,细胞凋亡率明显增加(P<0.01);细胞超微结构呈现典型的凋亡特征。结论:SBHL可通过组织细胞周期发展发挥抑制ME180细胞增殖的作用,SBHL还可诱导细胞发生凋亡。Objective:To experimental study the inhibiting effects of SBHL on the growth of cervical cancer cells and to investigate the mechanisms.Methods:The proliferation of ME180 cells was observed by MTT assasy; The changes of cells cycles and apoptosis of ME180 cells treated with SBHL were analyzed by agar gel electrophoresis and FCM assay;. The changes of the ultra structure of cells was observed by Transmission eleconmicrograph.Results:The proliferation of ME180 cells was inhibited after treating on SBHL for 24 h.The inhibiting effect increased granually following the concentration of SBHL(P<0.01).The percentage of cells in G1phase increased after the cells were treated by SBHL, indicating that SBHL arrested ME180 cells in G1phase; apoptosis was induced and apoptosis ratio increased obviously(P<0.01);typical apoptosis characteristic was present in the ultra structure of cells.Conclusion:The inhibiting effects of SBHL on the growth of cervical cancer cells was carried out by development of cell cycles; apoptosis was induced by SBHL .

关 键 词：澳洲茄胺盐酸盐(SBHL) 人宫颈癌 细胞凋亡 

分 类 号：R733.705[医药卫生—肿瘤]