COX-2基因沉默对人胃癌细胞系SGC-7901裸鼠成瘤的影响  

COX-2 gene silencing suppresses tumor formation of human gastric cancer cell lines SGC-7901 in nude mice

作　　者：汪步海[1] 钱伟[2] 朱良如[2] 侯晓华[2] 

机构地区：[1]东南大学附属中大医院肿瘤中心,江苏南京210009 [2]华中科技大学同济医学院协和医院消化内科,湖北武汉430022

出　　处：《现代医学》2007年第5期351-355,共5页Modern Medical Journal

摘　　要：目的研究COX-2基因沉默对人胃癌细胞系SGC-7901裸鼠成瘤的影响。方法构建靶向COX-2的短发夹状双链RNA(shRNA)的真核表达质粒转染人胃癌细胞系SGC-7901,采用RT-PCR和Westernblot分别从mRNA和蛋白质水平检测抑制效果。15只裸鼠随机分为3组,每组5只,皮下接种胃癌细胞。抑制组:接种转染抑制质粒的胃癌细胞;正常对照组:接种未转染的胃癌细胞;阴性对照组:接种转染阴性对照质粒的胃癌细胞。接种4周后比较各组裸鼠皮下形成瘤体的大体和病理情况并计算抑瘤率。结果转染靶向COX-2的shRNA抑制质粒后细胞中COX-2的表达被明显抑制,抑制率为70.42%。正常对照组和阴性对照组所有裸鼠均有瘤体形成,平均瘤质量分别为(0.49±0.017)g和(0.49±0.013)g。抑制组仅2只有瘤体形成,平均瘤质量(0.05±0.003)g,与正常对照组相比差别有统计学意义(P<0.01),抑瘤率为89.8%。结论运用RNA干扰可以高效特异地抑制人胃癌细胞系SGC-7901中COX-2的表达,COX-2被抑制后SGC-7901的生长和活性也被明显抑制。Objective To evaluate the inhibition effect of COX-2 gene silencing on tumor growth of human gastric cancer cell lines SGC-7901 in nude mice.Methods Eukaryotic expression plasmid of shRNA target to COX-2 was constructed.The effect of inhibition was detected by RT-PCT and Western blot after transfection with liposomes.Nude mice were divided into 3 groups,5 cases were involved in every group.Group inhibition:subcutaneous implant gastric cancer cells which COX-2 expression was suppressed.Group control:subcutaneous implant normal gastric cancer cells.Group sham:subcutaneous implant gastric cancer cells that were transfected sham plasmid.Morphological,histopathology and tumor inhibition rate were evaluated after 4 weeks.Results The expression of human COX-2 gene was suppressed specific and effectively by transfection shRNA plasmid into cells.The efficiency of inhibition was 70.42%.In group control and group sham tumor formation could be found in every case.Tumor tissue average weight of these two groups were(0.49±0.017)g and(0.49±0.013)g,while only in 2 cases(2/5)tumor formation could be found in group inhibition.Tumor tissue average weight of group inhibition was(0.05±0.003)g,tumor growth was inhibited significantly(P<0.01)and the inhibition rate was 89.8%.Conclusion The expression of COX-2 in human gastric cancer cell lines SGC-7901 can be inhibited specific and effectively by RNA interference that can result to tumor formation and growth suppressed obviously.

关 键 词：RNA干扰 COX-2 胃癌 细胞系 裸鼠 

分 类 号：R-33[医药卫生] R735.2