Senescence-like changes induced by expression of p21^(Waf1/Cipl)in NIH3T3 cell line  

Senescence-like changes induced by expression of p21^(Waf1/Cip1) in NIH3T3 cell line

作　　者：XI CHEN WEI ZHANG YUN FEI GAO XIAO QIN SU ZHONG HE ZHAI 

机构地区：[1]College of Life Sciences,Peking University,Beijing 100871,China [2]The Key Laboratory of Cell Proliferation and Regulation Biology of the Ministry of Education,College of Life Sciences,Beijing Normal University,Beijing 100875,China

出　　处：《Cell Research》2002年第4期229-233,共5页细胞研究(英文版)

基　　金：We thank Dr. Gordon Peters of Imperial CancerResearch Fund for providing p21(Waf1/Cip1) cDNA,Dr. Hong-Bing Shu of National Jewish Medica1and Reearch Center for pRetro-on-Bax and 293-10A1 packaging line. We also thank Drs. WeiTAO and Zhi Gang LU

摘　　要：P21Waf1/Cip1 is a potent cyclin-dependent kinase inhibitor. As a downstream mediator of p53, p21Waf1/Cip1 involves in cell cycle arrest, differentiation and apoptosis. Previous studies in human cells provided evidence for a link between p21Waf1/Cip1 and cellular senescence. While in murine cells, the role of p21Waf1/Cip1 is indefinite. We explored this issue using NIH3T3 cells with inducible p21Waf1/Cip1 expression. Induction of p21Waf1/Cip1 triggered G1 growth arrest, and NIH3T3-p21 cells exhibited morphologic features, such as enlarged and flattened cellular shape, specific to the senescence phenotype. We also showed that p21Waf1/Cip1-transduced NIH3T3 cells expressed β-galactosidase activity at pH 6.0, which is known to be a marker of senescence. Our results suggest that p21Waf1/Cip1 can also induce senescence-like changes in murine cells.

关 键 词：p21Wafl1/Cip1 SENESCENCE inducible expression cell cycle arrest. 

分 类 号：Q25[生物学—细胞生物学]