依曲替酸诱导皮肤鳞状细胞癌细胞系SCL-1凋亡及其机制的初步研究  

Inducement of acitretin to apoptosis of cutaneous squamous cell carcinoma cell line SCL-1 by upregulating Fas expression

作　　者：林秀英[1] 何春涤[1] 金鑫[1] 陈江[1] 王雅坤[1] 肖汀[1] 金光玉[1] 王凯波[1] 姜奕[1] 陈洪铎[1] 

机构地区：[1]中国医科大学附属第一医院皮肤科卫生部免疫皮肤病学重点实验室,辽宁沈阳110001

出　　处：《中华肿瘤防治杂志》2008年第9期644-647,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(30271198);教育部留学回国人员科研启动基金(2002247)

摘　　要：目的:探讨依曲替酸通过上调Fas基因表达诱导皮肤鳞状细胞癌(鳞癌)细胞系SCL-1细胞凋亡的作用。方法:以体外培养的人皮肤鳞癌细胞系SCL-1为研究对象,MTT法观察依曲替酸对SCL-1细胞的生长抑制作用,Annxin V-FITC/PI法检测早期凋亡,流式细胞仪检测细胞膜表面Fas的表达情况,实时荧光定量RT-PCR方法检测Fas mRNA的表达。结果:依曲替酸能显著抑制SCL-1细胞的生长,并具有浓度及时间依赖性。浓度为1×10-5mol/L的依曲替酸能诱导凋亡,上调细胞膜表面Fas表达水平及Fas mRNA水平。结论:依曲替酸能抑制皮肤鳞癌细胞生长及诱导凋亡,上调Fas抗原表达可能是其分子机制之一。OBJECTIVE:To investigate the effects of acitretin on the apoptosis of human cutaneous squamous cell carcinoma cell line SCL-1 by upregulating Fas expression. METHODS:Human cutaneous squamous cell carcinoma cell line SCL-1 was treated with acitretin. The cell proliferation was evaluated by MTT assay, and the early apoptosis was assessed with Annexin V-FITC/PI.Cell surface protein levels of Fas were examined by flow cytometry. The real-time quantitative RT-PCR was used to detect the expression of Fas mRNA. RE...

关 键 词：皮肤肿瘤 依曲替酸 细胞凋亡 癌 鳞状细胞 细胞系 肿瘤 

分 类 号：R739.5[医药卫生—肿瘤]