大鼠骨髓输注诱导小肠移植免疫耐受模型的建立  

Establishment of heterotopic small intestinal transplantation model after bone marrow infusion in inbred rat

作　　者：韩志锋[1] 李元新[1] 朱振新[1] 李秋荣[1] 

机构地区：[1]南京大学医学院临床学院(南京军区南京总医院)解放军普通外科研究所,江苏南京210002

出　　处：《肠外与肠内营养》2008年第2期77-79,共3页Parenteral & Enteral Nutrition

基　　金：江苏省自然科学基金资助项目(BK2004426)

摘　　要：目的:建立一种输注异基因特异性供鼠骨髓细胞后再行异位小肠移植的大鼠模型。方法:选取近交系Brown-Noway(BN)大鼠30只为供鼠;Lewis(LEW)大鼠15只为受鼠,2∶1随机配对。于术前麻醉处死BN大鼠,在无菌条件下提取股骨、胫骨的骨髓细胞,经LEWIS大鼠尾静脉输入,同时于术前经尾静脉,术后2、4、6 d经受鼠腹腔注射抗CD25单克隆抗体、CTLA-4Ig及抗CD154单克隆抗体,第8天行BN→LEW大鼠异位小肠移植。小肠移植术后第7天经内眦静脉采血0.5 mL,用流式细胞仪检测淋巴细胞嵌合率,并观察大鼠超急性排斥反应(GVHD)、生存期、体质量和一般状况。结果:小肠移植后15只大鼠均未观察到GVHD。小肠移植后存活>5 d为86.7%,>7 d为80.0%,>14 d为60.0%,最长存活>50 d。结论:输注异基因特异性供鼠骨髓细胞后再行大鼠异位小肠移植模型的建立,是进一步研究小肠移植特异性免疫耐受机制的模型之一。Objective: To establish the model of heterotopic small intestinal transplantation after bone marrow infusion in inbred rat.Methods: Thirty inbred Brown-Noway(BN)rats were choosed as donors and fifteen inbred Lewis(LEW) rats were choosed as receptors.Each animal of LEW rats received 2×108 bone marrow cells from BN rats through tail vein on day 0,and received anti-CD 25 monoclonal antibody,CTLA4Ig and anti-CD 154 monoclonal antibody by intraperitoneal injection(i.p.) on day 0(through tail vein),day 2,day 4,an...

关 键 词：小肠移植 骨髓细胞输注 嵌合 动物模型 大鼠 

分 类 号：R-332[医药卫生]