血管内皮生长因子在氩激光诱导大鼠脉络膜新生血管中的表达  

Expression and Significance of VEGF in Choroidal Neovascularization Induced by argon Laser in BN Rats

作　　者：赵鹏[1] 张喜梅[1] 贾亚丁[1] 

机构地区：[1]山西医科大学,山西太原030001

出　　处：《实用医技杂志》2008年第17期2177-2178,共2页Journal of Practical Medical Techniques

摘　　要：目的:探讨血管内皮生长因子(VEGF)在氩激光诱导BN大鼠脉络膜新生血管(CNV)形成过程中的表达及其意义。方法:BN大鼠18只,随机分成3组,每组6只,单眼视网膜氩激光光凝,诱导CNV形成。分别在光凝后1周、2周、3周摘除眼球,应用免疫组织化学技术对光凝区进行因子Ⅷ相关抗原(FⅧR:Ag)的检测以观测CNV形成,并检测CNV形成过程中VEGF蛋白的表达,分析其变化趋势。结果:FⅧR:Ag免疫组织化学检查结果显示,光凝后1周开始形成CNV,3周达到高峰。正常BN大鼠视网膜中,VEGF在视网膜神经节细胞层、内核层、色素上皮层、视网膜和脉络膜血管内皮细胞表达。视网膜光凝后,除上述部位外,VEGF在外核层和脉络膜的光凝损伤区阳性表达。光凝后1周~3周,光斑区内FⅧR:Ag、VEGF阳性染色密度逐渐增加(P<0.05),FⅧR:Ag与VEGF阳性染色密度正相关(r=0.83,P<0.05)。结论:CNV形成与VEGF表达正相关,VEGF表达增多可能是CNV形成和增殖的主要原因之一。Objective To study the expression of vessel endothelialgrowth factor(VEGF) in choroidal neovascularization (CNV), and to investigate its expression and significance on CNV induced by argon laser in BN rats. Methods Three groups of 18 BN rats were photocoagulated by argon laser in single eye. 1!week, 2 weeks, 3 weeks after photocoagulation, the expression of VEGF, and factorⅧ related antigen (FⅧR∶Ag) was observed by immunohis-tochemical method. Results FⅧR∶Ag positive staining cells formed vessels in the les...

关 键 词：脉络膜新生血管 血管内皮生长因子 Ⅷ因子相关抗原 

分 类 号：R779.63[医药卫生—眼科]